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Etoposide

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Chemical Structure| 33419-42-0 同义名 : 依托泊甘 ;VP-16;VP-16-213;Demethyl Epipodophyllotoxin Ethylidine Glucoside. epipodophyllotoxin. US brand names: Toposar. VePesid. Foreign brand name: Lastet. Abbreviation: EPEG Code names: VP16;VP-16-123;NSC 141540;EPE
CAS号 : 33419-42-0
货号 : A174646
分子式 : C29H32O13
纯度 : 98%
分子量 : 588.557
MDL号 : -
存储条件：

粉末 Keep in dark place,Sealed in dry,Room Temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 40 mg/mL(67.96 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：

5% DMSO+40% PEG 300+5% Tween 80+water 15 mg/mL

生物活性
靶点

  • Topo II
描述 Etoposide (VP-16; VP-16-213) is an anticancer chemotherapeutic agent. It functions by inhibiting topoisomerase II, thereby halting DNA replication. Etoposide induces cell cycle arrest, apoptosis, and autophagy [1].
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
A549 Growth Inhibition Assay 48 h IC50=241.9 ± 31.23 μM 24996136
A549 Growth Inhibition Assay 48 h  IC50=139.54 ± 7.05 μM 24793877
BGC-823 Growth Inhibition Assay 48 h  IC50=43.74 ± 5.13 μM 24793877
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.70mL

0.34mL

0.17mL

8.50mL

1.70mL

0.85mL

16.99mL

3.40mL

1.70mL
参考文献

[1]Lee KI, et al. Etoposide induces pancreatic β-cells cytotoxicity via the JNK/ERK/GSK-3 signaling-mediated mitochondria-dependent apoptosis pathway. Toxicol In Vitro. 2016 Jul 26. pii: S0887-2333(16)30147-3.

[2]Calvani M, det al. Etoposide-Anti-Human VEGF a new strategy against human melanoma cells expressing stem-like traits. Oncotarget. 2016 Jun 9. doi: 10.18632/oncotarget.9939.

[3]Fuchs, J., et al. Comparative activity of NSC 119875, NSC 109724, NSC 123127, NSC 241240, and etoposide in heterotransplanted hepatoblastoma. Cancer, 1998. 83(11): p. 2400-7.

[4]Cui D, et al. FBXW7 Confers Radiation Survival by Targeting p53 for Degradation.Cell Rep. 2020 Jan 14;30(2):497-509.e4.