产品说明书

Luminol

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Chemical Structure| 521-31-3 同义名 : 鲁米诺 ;Diogenes reagent;3-Aminophthalhydrazide;NSC 5064
CAS号 : 521-31-3
货号 : A166579
分子式 : C8H7N3O2
纯度 : 98%
分子量 : 177.16
MDL号 : MFCD00006890
存储条件：

粉末 Keep in dark place,Sealed in dry,Room Temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 30 mg/mL(169.34 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Luminol is a major probe for chemiluminescence (CL) and electrochemiluminescence (ECL) detection technologies in (bio)analysis. LCL (luminol chemiluminescence) is a very useful detection method due to its selectivity, simplicity, low cost, and high sensitivity. LCL has a dynamic range of applications, including quantification and detection of macro and micromolecules such as proteins, carbohydrates, DNA, and RNA. Luminol-based methods are used in environmental monitoring as biosensors, in the pharmaceutical industry for cellular localization and as biological tracers, and in reporter gene-based assays and several other immunoassays[1]. Moreover, a significant peak in chemiluminescence production in a particle-free system occurs between 5 and 15 min following exposure of cells to micromolar concentrations of luminol. The response is directly related to dose over a wide rane of luminol concentrations and can be inhibited by superoxide dismutase (90%), catalase (100%) and sodium azide (40%). The effect of luminol eliciting a peak in neutrophil chemiluminescence is mediated within intact cells rather than at the cell membrane. Luminol may produce a peak in chemiluminescence by stimulating very low levels of hexose monophosphate shunt activity and superoxide generation or it may simply amplify light production generated by the production of excited oxygen radicals resulting from surface interactions[2].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

5.64mL

1.13mL

0.56mL

28.22mL

5.64mL

2.82mL

56.45mL

11.29mL

5.64mL
参考文献

[1]Khan P, Idrees D, Moxley MA, Corbett JA, Ahmad F, von Figura G, Sly WS, Waheed A, Hassan MI. Luminol-based chemiluminescent signals: clinical and non-clinical application and future uses. Appl Biochem Biotechnol. 2014 May;173(2):333-55.

[2]Allred CD, Margetts J, Hill HR. Luminol-induced neutrophil chemiluminescence. Biochim Biophys Acta. 1980 Aug 13;631(2):380-5.