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MHY1485

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Chemical Structure| 326914-06-1 同义名 : 4,6-二吗啉-N-(4-硝基苯基)-1,3,5-三嗪-2-胺
CAS号 : 326914-06-1
货号 : A165303
分子式 : C17H21N7O4
纯度 : 99%+
分子量 : 387.393
MDL号 : MFCD00489974
存储条件：

粉末 Keep in dark place,Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 6 mg/mL(15.49 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Autophagy is a complicated process that consists of autophagosome biogenesis, maturation, and fusion with lysosomes, which can be induced by mtor inhibitor such as rapamycin. MHY1485 can activate mtor and suppress the basal autophagic flux in cells under starvation. It was shown that the LC3II/LC3I ratio and LC3II-positive vacuoles can be increased by simply treatment of MHY1485 2uM. However, compared with the group only with 10 nM bafilomycin A1 or 100uM chloroquine, treatment of MHY1485 2uM can decrease the LC3II protein level in Ac2F cells under starvation. These indicated that MHY1485 can suppress autophagic flux induced by starvation. Also the increase of both p-mTOR-Ser2448/mTOR ratio and the p4E-BP1-Thr37/46/4E-BP1 can be detected after treating cells with 2uM MHY1485, which indicated the activation of mTOR by MHY1485 [1]
作用机制 MHY1485 showed a high docking score with the ATP domain of mTOR.
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.58mL

0.52mL

0.26mL

12.91mL

2.58mL

1.29mL

25.81mL

5.16mL

2.58mL