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Hinokitiol

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Chemical Structure| 499-44-5 同义名 : β-桧木醇 ;β-Thujaplicin;NSC 18804;4-Isopropyltropolone
CAS号 : 499-44-5
货号 : A161005
分子式 : C10H12O2
纯度 : 99%
分子量 : 164.201
MDL号 : MFCD00040180
存储条件：

粉末 Sealed in dry,Room Temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(639.46 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Hinokitiol, a tropolone-related natural compound, is known to induce apoptosis and cell cycle arrest and has anti-inflammatory and anti-tumor activities. Hinokitiol may exert DNA demethylation by inhibiting the expression of DNMT1 (DNA methyltransferase 1) and UHRF1 (RING finger domain 1) in colon cancer cells[3]. In U87MG and T98G glioma cell lines, hinokitiol demonstrates a dose-dependent decrease in viability, with IC50 values of 316.5 ± 35.5 and 152.5 ± 25.3 µM, respectively. Hinokitiol represses ALDH activity and self-renewal ability in glioma stem cells, and inhibits in vitro oncogenicity. Hinokitiol also reduces Nrf2 expression in glioma stem cells in a dose-dependent manner[4]. Hinokitiol attenuates liver injury following HS/R (Haemorrhagic shock and resuscitation), partly through the inhibition of NF-κB activation[5]. Moreover, hinokitiol inhibited the migration of lung cancer A549 cells through several mechanisms, including the activation of caspases-9 and -3, induction of p53/Bax and antioxidant CAT (antioxidant enzymes catalase) and SOD (superoxide dismutase), and reduction of MMP-2 and -9 (matrix metalloproteinases) activities. It also induces cytochrome c expression[6].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

6.09mL

1.22mL

0.61mL

30.45mL

6.09mL

3.05mL

60.90mL

12.18mL

6.09mL
参考文献

[1]Lee JH, Moon JH, et al. SIRT1, a Class III histone deacetylase, regulates LPS-induced inflammation in human keratinocytes and mediates the anti-inflammatory effects of hinokitiol. J Invest Dermatol. 2017 Feb 28. pii: S0022-202X(17)31149-1.

[2]Seo JS, Choi YH, et al. Hinokitiol induces DNA demethylation via DNMT1 and UHRF1 inhibition in colon cancer cells. BMC Cell Biol. 2017 Feb 27;18(1):14.

[3]Seo JS, Choi YH, Moon JW, Kim HS, Park SH. Hinokitiol induces DNA demethylation via DNMT1 and UHRF1 inhibition in colon cancer cells. BMC Cell Biol. 2017;18(1):14.

[4]Ouyang WC, Liao YW, Chen PN, Lu KH, Yu CC, Hsieh PL. Hinokitiol suppresses cancer stemness and oncogenicity in glioma stem cells by Nrf2 regulation. Cancer Chemother Pharmacol. 2017;80(2):411‐419

[5]Lu WJ, Lin KH, Tseng MF, et al. New therapeutic strategy of hinokitiol in haemorrhagic shock-induced liver injury. J Cell Mol Med. 2019;23(3):1723‐1734

[6]Jayakumar T, Liu CH, Wu GY, et al. Hinokitiol Inhibits Migration of A549 Lung Cancer Cells via Suppression of MMPs and Induction of Antioxidant Enzymes and Apoptosis. Int J Mol Sci. 2018;19(4):939.