Solamargine

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Chemical Structure| 20311-51-7 同义名 : Solamargin;δ-Solanigrine;α-Solamargine, NSC 407810, Solamargine;α-Solamargine;NSC 407810
CAS号 : 20311-51-7
货号 : A156592
分子式 : C45H73NO15
纯度 : 98%
分子量 : 868.059
MDL号 : MFCD30207844
存储条件：

Pure form Keep in dark place,Inert atmosphere,2-8°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(120.96 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Solamargine, a derivative from the steroidal solasodine in Solanum species, exhibits anticancer activities in numerous types of cancer[3]. Solamargine inhibited the progression of GC (gastric cancer) by regulating lncNeat1_2 via the MAPK (mitogen-activated protein kinase) pathway[4]. Solamargine reduced HepG2 cell viability in a concentration-dependent manner. At 7.5μM solamargine decreased cell viability by less than 20% in HepG2 cells. A wound healing migration assay and Boyden chamber invasion assay showed that solamargine significantly inhibited in vitro migration and invasion of HepG2 cells. At the highest dose, solamargine decreased cell migration and invasion by more than 70% and 72% in HepG2 cells, respectively. Solamargine significantly inhibits migration and invasion of HepG2 cells by down-regulating MMP-2 and MMP-9 expression and activity[5]. Solamargine (15 μg/ml; 72 hours) predominantly stimulates the cells of the G2/M phase to apoptosis. Solamargine (72 hours) dramatically inhibits the proliferation of SMMC-7721 and HepG2 cells in a dose- and time-dependent manner. The IC50s are 9.21 and 19.88 μg/ml, respectively[6]. Solamargine activates the mitochondria-mediated apoptotic pathway in U2OS cells via both p53 transcription-dependent and -independent mechanisms[7]. Solamargine inhibited the viability of QBC939 cells in a dose-dependent manner. Furthermore, solamargine significantly induced the apoptosis of QBC939 cells and altered the mitochondrial membrane potential of cells[8].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.15mL

0.23mL

0.12mL

5.76mL

1.15mL

0.58mL

11.52mL

2.30mL

1.15mL
参考文献

[1]Sani IK, Marashi SH, et al. Solamargine inhibits migration and invasion of human hepatocellular carcinoma cells through down-regulation of matrix metalloproteinases 2 and 9 expression and activity. Toxicol In Vitro. 2015 Mar 27;29(5):893-900.

[2]Munari CC, de Oliveira PF, et al. Antiproliferative activity of Solanum lycocarpum alkaloidic extract and their constituents, solamargine and solasonine, in tumor cell lines. J Nat Med. 2014 Jan;68(1):236-41.

[3]Burger T, Mokoka T, Fouché G, Steenkamp P, Steenkamp V, Cordier W. Solamargine, a bioactive steroidal alkaloid isolated from Solanum aculeastrum induces non-selective cytotoxicity and P-glycoprotein inhibition. BMC Complement Altern Med. 2018 May 2;18(1):137

[4]Fu R, Wang X, Hu Y, Du H, Dong B, Ao S, Zhang L, Sun Z, Zhang L, Lv G, Ji J. Solamargine inhibits gastric cancer progression by regulating the expression of lncNEAT1_2 via the MAPK signaling pathway. Int J Oncol. 2019 May;54(5):1545-1554

[5]Sani IK, Marashi SH, Kalalinia F. Solamargine inhibits migration and invasion of human hepatocellular carcinoma cells through down-regulation of matrix metalloproteinases 2 and 9 expression and activity. Toxicol In Vitro. 2015 Aug;29(5):893-900

[6]Ding X, Zhu FS, Li M, Gao SG. Induction of apoptosis in human hepatoma SMMC-7721 cells by solamargine from Solanum nigrum L. J Ethnopharmacol. 2012 Jan 31;139(2):599-604

[7]Li X, Zhao Y, Wu WK, Liu S, Cui M, Lou H. Solamargine induces apoptosis associated with p53 transcription-dependent and transcription-independent pathways in human osteosarcoma U2OS cells. Life Sci. 2011 Feb 14;88(7-8):314-21

[8]Zhang X, Yan Z, Xu T, An Z, Chen W, Wang X, Huang M, Zhu F. Solamargine derived from Solanum nigrum induces apoptosis of human cholangiocarcinoma QBC939 cells. Oncol Lett. 2018 May;15(5):6329-6335