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Daunorubicin HCl

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Chemical Structure| 23541-50-6 同义名 : 柔红霉素盐酸盐 ;Daunomycin hydrochloride;RP 13057 hydrochloride;RP 13057;Ondena;NDC 0082-4155;Daunorubicin (hydrochloride);RP 13057 HCl;Rubidomycin HCl;Daunorubicin HCl;Rubidomycin hydrochloride
CAS号 : 23541-50-6
货号 : A148164
分子式 : C27H30ClNO10
纯度 : 98%
分子量 : 563.981
MDL号 : -
存储条件：

粉末 Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 50 mg/mL(88.66 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 30 mg/mL(53.19 mM)
动物实验配方：
生物活性
靶点

  • DNA synthesis

    DNA synthesis, Ki:20 nM

  • Topoisomerase

    DNA synthesis, Ki:20 nM
描述 Daunorubicin (Daunomycin) hydrochloride acts as a topoisomerase II inhibitor with strong anti-cancer properties. It disrupts both DNA and RNA synthesis and functions as a cytotoxic agent that reduces cancer cell survival while triggering apoptosis and necrosis. Additionally, Daunorubicin hydrochloride classifies as an anthracycline antibiotic. It is utilized in studying infections and various cancers, such as leukemia, non-Hodgkin lymphomas, Ewing's sarcoma, and Wilms' tumor[1][2][4][5].
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
african green monkey Vero cells Cytotoxic assay 3 days Cytotoxicity against african green monkey Vero cells after 3 days by MTS assay, IC50=2.5 μM 21094049
B16 mouse melanoma cells Cytotoxic assay Cytotoxicity that caused a 50% reduction in absorbance at 490 nm in B16 mouse melanoma cells using MTS assay, EC50=0.16 μM 11020285
CCRF-CEM (human lymphoblastic leukemic) cells Growth inhibition assay In vitro growth inhibitory activity against CCRF-CEM (human lymphoblastic leukemic) cells, ID50=0.04 μM 6951049
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.77mL

0.35mL

0.18mL

8.87mL

1.77mL

0.89mL

17.73mL

3.55mL

1.77mL
参考文献

[1]Lehmann M, et al. Activity of topoisomerase inhibitors daunorubicin, idarubicin, and aclarubicin in the Drosophila Somatic Mutation and Recombination Test. Environ Mol Mutagen. 2004;43(4):250-7.

[2]Dano K, et al. Inhibition of DNA and RNA synthesis by daunorubicin in sensitive and resistant Ehrlich ascites tumor cells in vitro. Cancer Res. 1972 Jun;32(6):1307-14.

[3]Svensson SP, et al. Melanin inhibits cytotoxic effects of Doxorubicin and Daunorubicin in MOLT 4 cells. Pigment Cell Res. 2003 Aug;16(4):351-4

[4]Gervasoni JE Jr, et al. An effective in vitro antitumor response against human pancreatic carcinoma with paclitaxel and Daunorubicin by induction of both necrosis and apoptosis. Anticancer Res. 2004 Sep-Oct;24(5A):2617-26

[5]Arozal W, et al. Telmisartan prevents the progression of renal injury in daunorubicin rats with the alteration of angiotensin II and endothelin-1 receptor expression associated with its PPAR-γ agonist actions. Toxicology. 2011 Jan 11;279(1-3):91-9.

[6]Emeline Bollaert, et al. MiR-15a-5p Confers Chemoresistance in Acute Myeloid Leukemia by Inhibiting Autophagy Induced by Daunorubicin. Int J Mol Sci. 2021 May 13;22(10):5153.

[7]Cheng Wu, et al. Doxorubicin suppresses chondrocyte differentiation by stimulating ROS production. Eur J Pharm Sci. 2021 Dec 1;167:106013.