产品说明书

Vitamin D3

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Chemical Structure| 67-97-0 同义名 : Cholecalciferol;Colecalciferol;Deparal;Vigorsan;Vigantol;Trivitan;Ricketon;Activated 7-dehydrocholesterol;Arachitol;Calciol;NSC 375571
CAS号 : 67-97-0
货号 : A146813
分子式 : C27H44O
纯度 : 98+%
分子量 : 384.638
MDL号 : MFCD00078131
存储条件:

粉末 Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月

溶解度 :

DMSO: 105 mg/mL(272.98 mM),配合低频超声助溶,注意:DMSO长时间开封后,会吸水并导致溶解能力下降,请避免使用长期开封的DMSO

动物实验配方:

30% PEG400+0.5% Tween80+5% propylene glycol+water 30 mg/mL suspension

生物活性
描述 Vitamin D3 (Cholecalciferol) is a naturally occuring form of vitamin D. Cholecalciferol induces cell differentiation and prevents proliferation of cancer cells. Cholecalciferol (2-10 μM; 24-48 hours) exhibits anti-proliferative effects in a dose- and time- dependent manner. Maximal reduction of viability post treatment of 62% (IK), 52% (RL-95-2), and 55% (Hec-1A) occurrs by 72 h of treatment with 10 μM VD3. but 24-hour exposure lacks significant reduction in viable cells[3]. Active vitamin D3(calcitriol)and its derivatives(alfacalcidol, eldecalcitol), and vitamin K2(menatetrenone)are used as the therapeutic medicines for osteoporosis in Japan[4]. Elevated calcium levels were induced in mice by pre-treatment with cholecalciferol (vitamin D3; V.D3). Exposure to V.D3 alone had no effect on plasma markers of liver or kidney damage or on total antioxidant power or lipid peroxidation. The potentiating effect of V.D3 was positively correlated with elevation of hepatic calcium levels[5]. Supplementation with vitamin D3 and calcium reduces the risk of hip fractures and other nonvertebral fractures among elderly women[6].
临床研究
NCT号 适应症或疾病 临床期 招募状态 预计完成时间 地点
NCT02076750 Inflammatory Bowel Diseases ... 展开 >> Skin Pigmentation 收起 << Phase 1 Phase 2 Completed - United States, Georgia ... 展开 >> Children's Healthcare of Atlanta, Egleston Children's Hospital Atlanta, Georgia, United States, 30322 Emory Children's Center Atlanta, Georgia, United States, 30322 收起 <<
NCT01608451 Locally Advanced Breast Cancer... 展开 >> and Large Operable Breast Cancer 收起 << Phase 3 Active, not recruiting December 2020 India ... 展开 >> Tata Memorial Hospital Mumbai, Maharashtra, India, 400012 收起 <<
NCT03754712 MDD Not Applicable Recruiting February 28, 2019 Bangladesh ... 展开 >> BSMMU Recruiting Dhaka, Bangladesh Contact: Tanzina Afrin, MBBS    01796372338    Tanzina.afrin800@gmail.com    Contact: Sultanul Arifin, MBBS    01723836535    Sam35th@gmail.com    Principal Investigator: Tanzina Afrin, MBBS 收起 <<
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.60mL

0.52mL

0.26mL

13.00mL

2.60mL

1.30mL

26.00mL

5.20mL

2.60mL

参考文献

[1]Ousley AM, Castillo HS, et al. A human vitamin D receptor mutant activated by cholecalciferol. J Steroid Biochem Mol Biol. 2011 Jul;125(3-5):202-10.

[2]Diesing D, Cordes T, et al. Vitamin D--metabolism in the human breast cancer cell line MCF-7. Anticancer Res. 2006 Jul-Aug;26(4A):2755-9.

[3]Bergadà L, Pallares J, Maria Vittoria A, Cardus A, Santacana M, Valls J, Cao G, Fernàndez E, Dolcet X, Dusso AS, Matias-Guiu X. Role of local bioactivation of vitamin D by CYP27A1 and CYP2R1 in the control of cell growth in normal endometrium and endometrial carcinoma. Lab Invest. 2014 Jun;94(6):608-22

[4]Tsugawa N. [Clinical Pharmacokinetics of Active Vitamin D3 and its derivatives, and Vitamin K2(Menatetrenone).]. Clin Calcium. 2016;26(11):1547-1558. Japanese

[5]Yoshioka H, Usuda H, Miura N, Fukuishi N, Nonogaki T, Onosaka S. Vitamin D3-induced hypercalcemia increases carbon tetrachloride-induced hepatotoxicity through elevated oxidative stress in mice. PLoS One. 2017 Apr 27;12(4):e0176524

[6]Chapuy MC, Arlot ME, Duboeuf F, Brun J, Crouzet B, Arnaud S, Delmas PD, Meunier PJ. Vitamin D3 and calcium to prevent hip fractures in elderly women. N Engl J Med. 1992 Dec 3;327(23):1637-42