DOTMA

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Chemical Structure| 104872-42-6 同义名 : -
CAS号 : 104872-42-6
货号 : A1444827
分子式 : C42H84ClNO2
纯度 : 98%+
分子量 : 670.575
MDL号 : N/A
存储条件：

Pure form Inert atmosphere,Room Temperature

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 12 mg/mL(17.9 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 DOTMA is a cationic lipid utilized as a non-viral vector in gene therapy, serving as a component in liposomes for encapsulating siRNA, microRNA, and oligonucleotides, and for in vitro gene transfection. DOTMA enhances the interaction between liposomes and cell membranes by inducing a positive charge, showing effective gene transfection both in vitro and in vivo [1][2][3][4][5].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.49mL

0.30mL

0.15mL

7.46mL

1.49mL

0.75mL

14.91mL

2.98mL

1.49mL
参考文献

[1]Aime S, et al. Properties, solution state behavior, and crystal structures of chelates of DOTMA. Inorg Chem. 2011;50(17):7955-7965.

[2]Bhavsar D, et al. Translational siRNA therapeutics using liposomal carriers: prospects & challenges. Curr Gene Ther. 2012;12(4):315-332.

[3]Ren T, et al. Structural basis of DOTMA for its high intravenous transfection activity in mouse. Gene Ther. 2000;7(9):764-768.

[4]Wang X, et al. Enhanced hepatic delivery of siRNA and microRNA using oleic acid based lipid nanoparticle formulations. J Control Release. 2013 Dec 28;172(3):690-8.

[5]Huang QD, et al. Cyclen-based cationic lipids for highly efficient gene delivery towards tumor cells. PLoS One. 2011;6(8):e23134.