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Limonin

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Chemical Structure| 1180-71-8 同义名 : 吴茱萸内酯 ;Limonoic acid 3,19:16,17 dilactone;di-δ-lactone Limonoic Acid;Obaculactone.;NSC 36508;Limonine;Evodin;Dictamnolactone;Citro
CAS号 : 1180-71-8
货号 : A142805
分子式 : C26H30O8
纯度 : 98%
分子量 : 470.512
MDL号 : MFCD00075922
存储条件：

粉末 Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 40 mg/mL(85.01 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
靶点

  • HIV Protease
描述 The HIV-1 protease belongs to the family of aspartyl protease. It cleaves the newly synthesized polyproteins,which is the vital step to create the mature protein components of an infectious HIV-1 virus[3].Limonin s a natural tetracyclic triterpenoid compound, it inhibits HIV-1 replication in culturedf mon-cytes, macrophages, and mononuclear cells ,by inhibition of HIV-1 protease activity.[4]. Limonin can effectively inhibit the replication of human immunodeficiency virus-1 (HIV-1) (EC50 = 60.0 µM) in a dose-dependent manner, and its mechanism may be related to the inhibition of HIV-1 protease activity.[4]. the HIV-1 gag expression was completely inhibited by limonin with a concentration of 1 µg/mL. Hence, limonin had effective antiretroviral activity against HTLV-1 and HIV-1 infection i in vitro .[5].Limonin (50 mg/kg) has excellent antioxidant and therapeutic effects on N-nitroethylenediamine (DEN)-induced hepatocarcinoma rats by suppressing lipid peroxidation (LPO) and oxidative stress-mediated free radicals generation, and through modulating antioxidants’ defense mechanism[6]. Limonin significantly decreased the levels of tumor necrosis factor-α (TNF-α), interleukin (IL-1β and IL-6), and inhibited the expression of inflammatory factors in lipopolysaccharide (LPS)-induced acute lung injury mice[7].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.13mL

0.43mL

0.21mL

10.63mL

2.13mL

1.06mL

21.25mL

4.25mL

2.13mL
参考文献

[1]Battinelli L, Mengoni F, et al. Effect of limonin and nomilin on HIV-1 replication on infected human mononuclear cells. Planta Med. 2003 Oct;69(10):910-3.

[2]Matsuda H, Yoshikawa M, et al. Antinociceptive and anti-inflammatory activities of limonin isolated from the fruits of Evodia rutaecarpa var. bodinieri. Planta Med. 1998 May;64(4):339-42.

[3] Davies, D. R. The structure and function of the aspartic proteinases. Annu. Rev. Biophys. Biophys. Chem. 19, 189–215,

[4] Battinelli, L.; Mengoni, F.; Lichtner, M.; Mazzanti, G.; Saija, A.; Mastroianni, C.M.; Vullo, V. Effect of limonin and nomilin on HIV-1 replication on infected human mononuclear cells. Planta Med. 2003, 69, 910–913.

[5] Balestrieri, E.; Pizzimenti, F.; Ferlazzo, A.; Giofrè, S.V.; Iannazzo, D.; Piperno, A.; Romeo, R.; Chiacchio, M.A.;Mastino, A.; Macchi, B. Antiviral activity of seed extract from Citrus bergamia towards human retroviruses.Bioorg. Med. Chem. 2011, 19, 2084–2089.

[6] Langeswaran, K.; Kumar, S.G.; Perumal, S.; Revathy, R.; Balasubramaniam, M.P. Limonin–A citrus limonoid, establish anticancer potential by stabilizing lipid peroxidation and antioxidant status against N-nitrosodiethylamine induced experimental hepatocellular carcinoma. Biomed. Prev. Nutr. 2013, 3, 165–171.

[7] Wang, D.; Zhang, H.H.; Fang, J.; Zhong, Y.S.; Yu, C.H. Effects of limoninon on LPS-induced acute lung injury in mice. Chin. J. Clinc. Pharmacol. Therapeut. 2018, 23, 8–12. (In Chinese)