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Solasodine

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Chemical Structure| 126-17-0 同义名 : 澳洲茄铵 ;Solasodin;Solancarpidine;(−)-Solasodine;Solasod-5-en-3β-ol;NSC 178260;NSC 179187;Salasdine;Salasodine;Purapuridine
CAS号 : 126-17-0
货号 : A142284
分子式 : C27H43NO2
纯度 : 99%+
分子量 : 413.636
MDL号 : MFCD00037844
存储条件：

粉末 Keep in dark place,Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 1.5 mg/mL(3.63 mM)，配合低频超声，水浴加热至45℃，并调节pH至5，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

无水乙醇: 20 mg/mL(48.35 mM)，配合低频超声助溶，注意：无水乙醇开封后，易挥发，也会吸收空气中的水分，导致溶解能力下降，请避免使用开封较久的乙醇
动物实验配方：
生物活性
描述 Solasodine is a poisonous alkaloid chemical compound that occurs in plants of the Solanaceae family. Solasodine has neuroprotective, antifungal, hypotensive, anticancer, antiatherosclerotic, antiandrogenic and anti-inflammatory activities. Solasodine induces strong expression of the different neuronal markers studied, including βIII-tubulin, synaptophysin, MAP2, ChAT, and neuroblast marker doublecortin. Solasodine (375 μM; i.c.v.; for 2 weeks) treatment results a significant increase in bromodeoxyuridine uptake by cells of the ependymal layer, subventricular zone, and cortex that co-localized with doublecortin immunostaining[3]. Intraperitoneal (i.p.) injection of solasodine (25 mg/kg) significantly delayed latency of hind limb tonic extensor (HLTE) phase in the PCT-induced convulsions. In the MES model, solasodine significantly reduced duration of HLTE at 25, 50, and 100 mg/kg, i.p. in a dose-dependent manner. Treatment of solasodine (25, 50, and 100 mg/kg, i.p.) significantly potentiated thiopental-provoked sleep in a dose-dependent manner[4]. CD133, CD44, Nanog, Oct-4 and Sox-2 were inhibited by solasodine to reverse stemness and similar mechanism was stimulated in vivo. Moreover, solasodine attenuated TGF-β1-induced EMT and decreased MMPs while in vivo study showed the same trend[5]. Inhibition of A549 cell invasion by solasodine may be, at least in part, through blocking MMP expression. Solasodine also reduces PI3K/Akt signaling pathways and downregulates expression of miR-21[6].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.42mL

0.48mL

0.24mL

12.09mL

2.42mL

1.21mL

24.18mL

4.84mL

2.42mL
参考文献

[1]Lecanu L, Hashim AI, et al. The naturally occurring steroid solasodine induces neurogenesis in vitro and in vivo. Neuroscience. 2011 Jun 2;183:251-64.

[2]Chauhan K, Sheth N, et al. Anticonvulsant activity of solasodine isolated from Solanum sisymbriifolium fruits in rodents. Pharm Biol. 2011 Feb;49(2):194-9.

[3]Lecanu L, Hashim AI, McCourty A, Giscos-Douriez I, Dinca I, Yao W, Vicini S, Szabo G, Erdélyi F, Greeson J, Papadopoulos V. The naturally occurring steroid solasodine induces neurogenesis in vitro and in vivo. Neuroscience. 2011 Jun 2;183:251-64

[4]Chauhan K, Sheth N, Ranpariya V, Parmar S. Anticonvulsant activity of solasodine isolated from Solanum sisymbriifolium fruits in rodents. Pharm Biol. 2011 Feb;49(2):194-9

[5]Zhuang YW, Wu CE, Zhou JY, Zhao ZM, Liu CL, Shen JY, Cai H, Liu SL. Solasodine reverses stemness and epithelial-mesenchymal transition in human colorectal cancer. Biochem Biophys Res Commun. 2018 Oct 28;505(2):485-491

[6]Shen KH, Hung JH, Chang CW, Weng YT, Wu MJ, Chen PS. Solasodine inhibits invasion of human lung cancer cell through downregulation of miR-21 and MMPs expression. Chem Biol Interact. 2017 Apr 25;268:129-135