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Vacuolin-1

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Chemical Structure| 351986-85-1 同义名 : -
CAS号 : 351986-85-1
货号 : A1359469
分子式 : C26H24IN7O
纯度 : 99%+
分子量 : 577.419
MDL号 : MFCD00321410
存储条件：

粉末 Keep in dark place,Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 9 mg/mL(15.59 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Exocytic fusion of lysosomes triggered by plasma membrane damage is proposed to be the major source of the membrane required for resealing. Vacuolin-1 is a potent and cell-permeable lysosomal exocytosis inhibitor which blocks the Ca2+-dependent exocytosis of lysosomes and prevents the release of lysosomal content without affecting the process of resealing[1]. Vacuolin-1 is also a potent inhibitor of the phosphatidylinositol phosphate kinase, PIKfyve with a Kd of 32 nM. In H4 cells expressing mCherry-GFP LC3, vacuolin-1 (3 μM; 24 hr) induced the accumulation of AcGFP- and mCherry-positive puncta, indicating that vacuolin-1 inhibits autophagy by inhibiting the degradation step of autophagosome. In HeLa cells, vacuolin-1 (3 μM; 24 hr) decreased mature cathepsin D levels and increased immature cathepsin D levels, suggesting that vacuolin-1 impairs the endosomal trafficking of de novo synthesized lysosomal enzymes, which in turn results in the suppression of the lysosomal degradation pathway. Moreover, vacuolin-1 treatment induced enlarged M6PR-positive vesicle formation (M6PR is a marker of transport between Golgi bodies and lysosomes) in HeLa cells, further suggesting that vacuolin-1 inhibits autophagy by inhibiting the maturation of lysosomes[2].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.73mL

0.35mL

0.17mL

8.66mL

1.73mL

0.87mL

17.32mL

3.46mL

1.73mL
参考文献

[1]Cerny J, et al. The small chemical vacuolin-1 inhibits Ca(2+)-dependent lysosomal exocytosis but not cell resealing. EMBO Rep. 2004 Sep;5(9):883-8

[2]Sano O, et al. Vacuolin-1 inhibits autophagy by impairing lysosomal maturation via PIKfyve inhibition. FEBS Lett. 2016 Jun;590(11):1576-85