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Retinoic acid

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Chemical Structure| 302-79-4 同义名 : 维生素A酸 ;Vitamin A acid;all-trans-Retinoic acid;ATRA;Vitinoin;StievaA Forte;StievaA;RetisolA;Eudyna;EpiAberel;Dermairol;RetinA MICRO;RetinA;Renova;Avita;Aknoten;Vitamin A Acid. US brand names: Aberel;tretinoinum;trans vitamin A acid;trans retinoic acid;TRA;betaretinoic acid;alltrans vitamin A acid;RA;NSC 122758;NSC 122578;Tretinoin
CAS号 : 302-79-4
货号 : A126336
分子式 : C20H28O2
纯度 : 98%
分子量 : 300.44
MDL号 : MFCD00001551
存储条件：

粉末 Keep in dark place,Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 50 mg/mL(166.42 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Retinoic acid receptors (RARs) are members of the super‐family of nuclear hormone receptors, which directly regulate the transcription of their target genes in the nucleus. Retinoic acid, the active metabolite of vitamin A, is a ligand for retinoic acid receptor (RAR) and retinoid X receptor (RXR). It has pleiotropic effects on growth, differentiation, proliferation, and development, and determines embryonic pattern formation and inhibits tumor growth. Addition of tretinoin significantly suppressed the remarkably elevated MMP-13 mRNA expression in the keloid‐derived dermal fibroblasts, with the peak suppression at 12h[3]. Tretinoin also prevented the cytotoxicity of H2O2 in cultured human mesangial cells by increasing both the catalase activity and the reduced glutathione content in dose- and time-dependent manner[4]. Retinoic acid at 10 μM inhibited the migration of Aspc-1 and Panc-1 cells indirectly through its inhibitory effects on cancer associated fibroblasts (CAFs) and inhibited epithelial-mesenchymal transition of tumor cells by decreasing IL-6 secretion of CAFs[5]. Neural stem cells (NSCs) treated with 1 µM retinoic acid for 4 days showed increased HRas and Tuj1 levels together with the increase in the active HRas level, which was required for NSC differentiation into neurons and murine brain development[6].
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
COS7 cells Function assay 16 h Activity at human RARgamma ligand binding domain expressed in COS7 cells co-transfected with Gal4-DBD assessed as transcriptional activation after 16 hrs by Gal4 response element-driven luciferase reporter gene assay, EC50=0.0002 μM 19058965
CV-1 cells Function assay Transcriptional activation in CV-1 cells expressing Retinoic acid receptor RAR gamma, EC50=0.0007 μM 7490725
HL-60 cells Function assay Induction of transglutaminase (TGase) activity in HL-60 cells, EC50=0.005 μM 8784454
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.33mL

0.67mL

0.33mL

16.64mL

3.33mL

1.66mL

33.28mL

6.66mL

3.33mL
参考文献

[1]Uchida G, Yoshimura K, et al. Tretinoin reverses upregulation of matrix metalloproteinase-13 in human keloid-derived fibroblasts. Exp Dermatol. 2003;12 Suppl 2:35-42.

[2]Manzano VM, Puyol MR, et al. Tretinoin prevents age-related renal changes and stimulates antioxidant defenses in cultured renal mesangial cells. J Pharmacol Exp Ther. 1999 Apr;289(1):123-32.

[3]Uchida G, Yoshimura K, Kitano Y, Okazaki M, Harii K. Tretinoin reverses upregulation of matrix metalloproteinase-13 in human keloid-derived fibroblasts. Exp Dermatol. 2003;12 Suppl 2:35-42. doi: 10.1034/j.1600-0625.12.s2.6.x. PMID: 14756522.

[4]Manzano VM, Puyol MR, Puyol DR, Cazaña FJ. Tretinoin prevents age-related renal changes and stimulates antioxidant defenses in cultured renal mesangial cells. J Pharmacol Exp Ther. 1999 Apr;289(1):123-32. PMID: 10086995.

[5]Guan J, Zhang H, Wen Z, Gu Y, Cheng Y, Sun Y, Zhang T, Jia C, Lu Z, Chen J. Retinoic acid inhibits pancreatic cancer cell migration and EMT through the downregulation of IL-6 in cancer associated fibroblast cells. Cancer Lett. 2014 Apr 1;345(1):132-9. doi: 10.1016/j.canlet.2013.12.006. Epub 2013 Dec 12. PMID: 24334138.

[6]Park JC, Jeong WJ, Kim MY, Min D, Choi KY. Retinoic-acid-mediated HRas stabilization induces neuronal differentiation of neural stem cells during brain development. J Cell Sci. 2016 Aug 1;129(15):2997-3007. doi: 10.1242/jcs.184366. Epub 2016 May 16. PMID: 27185863.