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Ned 19

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Chemical Structure| 874374-25-1 同义名 : -
CAS号 : 874374-25-1
货号 : A1257146
分子式 : C30H31FN4O3
纯度 : 99%+
分子量 : 514.591
MDL号 : MFCD02057367
存储条件：

粉末 Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 120 mg/mL(233.2 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Ned 19 is a selective, membrane-permeable, non-competitive NAADP antagonist that inhibits NAADP-mediated Ca2+ signalling with an IC50 value of 65 nM[1] .Ned 19 strongly inhibits tumour growth, angiogenesis and lung metastasis in mice.Ned 19 acts in the concentration range of 25-100 μM for 24-72 hours, decreases the percentage of B16 cells in S phase, increases the percentage in G0/G1 phase, induces apoptosis in a concentration- and time-dependent manner, and reduces cell proliferation.Ned 19 decreases N-adhesin expression and increases E-adhesin expression and affects cell migration behaviour[2].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.94mL

0.39mL

0.19mL

9.72mL

1.94mL

0.97mL

19.43mL

3.89mL

1.94mL
参考文献

[1]Rosen D, et al. Analogues of the nicotinic acid adenine dinucleotide phosphate (NAADP) antagonistNed-19 indicate two binding sites on the NAADP receptor. J Biol Chem. 2009 Dec 11;284(50):34930-4.

[2]Annarita Favia, et al. NAADP-Dependent Ca2+ Signaling Controls Melanoma Progression, Metastatic Dissemination and Neoangiogenesis. Sci Rep. 2016; 6: 18925.