产品说明书
Jedi2
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同义名 : | - |
| CAS号 : | 651005-90-2 | |
| 货号 : | A1235231 | |
| 分子式 : | C10H8O3S | |
| 纯度 : | 98% | |
| 分子量 : | 208.234 | |
| MDL号 : | MFCD01569561 | |
| 存储条件： |
粉末 Keep in dark place,Sealed in dry,2-8°C 液体 -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 50 mg/mL(240.11 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO |
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| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | Piezo channels are mechanosensitive ion channels. Piezo1 is primarily expressed in nonsensory tissues, whereas Piezo2 is predominantly found in sensory tissues, including dorsal root ganglion (DRG) neurons[1].Jedi2 is a Piezo1 activator, but not a specific Piezo2 activator. Jedi2 binds to the mouse Piezo1 proteins with a Kd of 2770 mm. Jedi2 evokes Piezo1-mediated Ca2+ influx. Jedi2 (200 μM) specifically elicits dose-dependent responses in mPiezo1-transfected cells, but not in mPiezo2- or vector-transfected cells. Jedi2 (1 mM) causes an increase in Ca2+ in mCherry-positive HEK293T cells transfected with the mPiezo1-mCherry construct, which also responded to Yoda1 (30 μM). Jedi-induced activation of Piezo1 requires the key mechanotransduction components, including the two extracellular loops in the distal blade and the two leucine residues in the proximal end of the beam[2]. Piezo1 protein was present in endothelium and PVAT of rat aortic and in PVAT of mesenteric artery.The Piezo1 agonists Yoda1 and Jedi2 (1 nM - 10 µM) did not stimulate aortic contraction [max < 10% phenylephrine (PE) 10 µM contraction] or relaxation in tissues + or -PVAT. Depolarizing the aorta by modestly elevated extracellular K+ did not unmask aortic contraction to Yoda1 (max <10% PE 10 µM contraction)[3]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
4.80mL 0.96mL 0.48mL |
24.01mL 4.80mL 2.40mL |
48.02mL 9.60mL 4.80mL |
| 参考文献 |
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