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GW284543

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Chemical Structure| 790186-68-4 同义名 : UNC10225170
CAS号 : 790186-68-4
货号 : A1176981
分子式 : C23H20N2O3
纯度 : 99%+
分子量 : 372.416
MDL号 : MFCD32067894
存储条件：

粉末 Keep in dark place,Sealed in dry,Room Temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 120 mg/mL(322.22 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 The KRAS gene is an oncogene that encodes a GTPase transductor protein, K-Ras. The mutant KRAS regulates Myc, a transcription factor in MAPK/ERK signaling pathway, via ERK1/2-dependent and independent mechanisms in pancreatic ductal adenocarcinoma. MEK5 is a key component in MAPK pathway that phosphorylates and activates non-classical MAPK ERK5. GW284543, also known as UNC10225170, is a 4-anilinoquin(az)oline chemotype that selectively inhibits MEK5. Treatment of MIA PaCa-2 cells with GW284543 (10 and 20 μM) for 6 h decreased the expression of endogenous Myc protein in a concentration-dependent manner. The GW284543 treatment (10 and 20 μM) also dose-dependently inhibited MEK5 in MIA PaCa-2 cells, as indicated by the reduction in phosphorylated ERK5[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.69mL

0.54mL

0.27mL

13.43mL

2.69mL

1.34mL

26.85mL

5.37mL

2.69mL
参考文献

[1]Vaseva AV, Blake DR, Gilbert TSK, et al. KRAS Suppression-Induced Degradation of MYC Is Antagonized by a MEK5-ERK5 Compensatory Mechanism. Cancer Cell. 2018;34(5):807-822.e7.