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ZCL278

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Chemical Structure| 587841-73-4 同义名 : -
CAS号 : 587841-73-4
货号 : A117081
分子式 : C21H19BrClN5O4S2
纯度 : 98%
分子量 : 584.894
MDL号 : MFCD03623090
存储条件：

粉末 Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 50 mg/mL(85.49 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
靶点

  • Cdc42-subclass

    Cdc42 GTPase, Kd:11.4 μM
描述 ZCL278 is a potent, cell-permeable inhibitor specific to Cdc42, suppressing actin-based cellular functions such as Golgi organization and cell motility. In Swiss 3T3 fibroblast cultures, ZCL278 disrupts microspike formation and GM130-docked Golgi structures, prominent Cdc42-mediated subcellular events. It reduces perinuclear accumulation of active Cdc42 compared to NSC23766, a selective Rac inhibitor. ZCL278 also inhibits Cdc42-mediated neuronal branching, growth cone dynamics, actin-based motility, and migration in PC-3 metastatic prostate cancer cells without impacting cell viability [1]. ZCL278 acts as an entry inhibitor for Junin virus (JUNV), vesicular stomatitis virus, lymphocytic choriomeningitis virus, and dengue virus, but not for nonenveloped poliovirus. It efficiently inhibits chemically induced filopodium formation, a process dependent on Cdc42 activity. Dose-response experiments in Vero cells show that ZCL278, up to 200 μM, is not toxic but inhibits JUNV with an IC50 of ~14 μM, measured by flow cytometry [2].
作用机制 ZCL278 is a small molecule that binds to Cdc42 residues to target the interaction between Cdc42 and intersectin and inhibits Cdc42-mediated cellular processes.
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.71mL

0.34mL

0.17mL

8.55mL

1.71mL

0.85mL

17.10mL

3.42mL

1.71mL
参考文献

[1]Friesland A, et al. Small molecule targeting Cdc42-intersectin interaction disrupts Golgi organization and suppresses cell motility. Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1261-6.

[2]Chou YY, et al. Identification and Characterization of a Novel Broad-Spectrum Virus Entry Inhibitor. J Virol. 2016 Apr 14;90(9):4494-510.