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FITC-Dextran

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Chemical Structure| 60842-46-8 同义名 : 荧光素异硫氰酸酯-葡聚糖
CAS号 : 60842-46-8
货号 : A1149843
分子式 : -
纯度 : Average MW 3,000-5,000
分子量 : 0
MDL号 : MFCD00131092
存储条件:

粉末 Keep in dark place,Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月

溶解度 :

H2O: 15 mg/mL,配合低频超声助溶

DMSO: 12 mg/mL,配合低频超声,并水浴加热至45℃助溶,注意:DMSO长时间开封后,会吸水并导致溶解能力下降,请避免使用长期开封的DMSO

动物实验配方:
生物活性
描述 FITC-Dextran (MW 10000) serves as a fluorescent tracer conjugated with fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). It functions as a marker to detect heat shock-induced cell damage and investigate both early and late stages of apoptosis. Additionally, FITC-Dextran (MW 10000) is utilized in cell permeability assessments, including studies on blood-brain barrier permeability and determination of the degree of blood-brain barrier disruption[1][2][3]. FITC-Dextran (MW 4000) functions as a fluorescent tracer conjugated with fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). It serves as a marker for detecting heat shock-induced cell damage and investigating both early and late stages of apoptosis. Additionally, FITC-Dextran (MW 4000) is utilized in cell permeability assessments, including studies on blood-brain barrier permeability and determination of the degree of blood-brain barrier disruption[1][2][3]. FITC-Dextran (MW 3000-5000) serves as a fluorescent tracer conjugated with fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). It functions as a marker to detect heat shock-induced cell damage and investigate both early and late stages of apoptosis. Additionally, FITC-Dextran (MW 3000-5000) is utilized in cell permeability assessments, including studies on blood-brain barrier permeability and determination of the degree of blood-brain barrier disruption[1][2][3]. FITC-Dextran (MW 40000) functions as a fluorescent tracer conjugated with fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). It serves as a marker to detect heat shock-induced cell damage and investigate both early and late stages of apoptosis. Additionally, FITC-Dextran (MW 40000) is utilized in cell permeability assessments, including studies on blood-brain barrier permeability and determination of the degree of blood-brain barrier disruption[1][2][3]. FITC-Dextran (MW 150000) serves as a fluorescent tracer conjugated with fluorescein isothiocyanate (FITC) dextran (Ex=491 nm; Em=518 nm). It functions as a marker to detect heat shock-induced cell damage and investigate both early and late stages of apoptosis. Additionally, FITC-Dextran (MW 150000) is employed in perfusion studies in animals or in fluorescence microlymphography to study processes affecting the permeability of the blood-brain barrier (BBB)[6]. FITC-Dextran (MW 150000) serves as a fluorescent tracer for investigating cell permeability[7].
参考文献

[1]Moumaris M, et al. Fluorescein isothiocyanate-dextran can track apoptosis and necrosis induced by heat shock of peripheral blood mononuclear cells and HeLa cells[J]. Open Biological Sciences Journal, 2015, 1(1).

[2]Natarajan R, et al. Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability. Curr Protoc Neurosci. 2017 Apr 10;79:9.58.1-9.58.15.

[3]Eriksson I, et al. Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells. Methods Mol Biol. 2017;1594:179-189.

[4]Bolliner A,et al.,Fluorescence microlymphography: diagnostic potential in lymphedema and basis for the measurement of lymphatic pressure and flow velocity. Lymphology. 2007 Jun;40(2):52-62.

[5]Ishii T, et al., Accumulation of macromolecules in brain parenchyma in acute phase of cerebral infarction/reperfusion. Brain Res. 2010 Mar 19;1321:164-8.