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Phytohemagglutinin

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Chemical Structure| 9008-97-3 同义名 : 植物血球凝集素 ;PHA-M;Phaseolus vulgaris agglutinin;PHA
CAS号 : 9008-97-3
货号 : A1145366
分子式 : -
纯度 : lyophilized
分子量 : 0
MDL号 : MFCD00165279
存储条件：

粉末 Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

H2O: 20 mg/mL，配合低频超声助溶
动物实验配方：
生物活性
描述 Phytohemagglutinin M (PHAM) has been purified from the commercial mixture of proteins produced by Phaseolus vulgaris, using a Sepharose-thyroglobulin column[4]. 17 µM ENDO (endosulfan) inhibited phytohemagglutinin-M (PHA)-induced human PBMC (peripheral blood mononuclear cells ) proliferation[5]. Proliferative activity of lymphocytes when stimulated with pokeweed mitogen, Concanavalin A and Phytohemagglutinin, M form did not differ depending on the dietary protein concentration and quality[6]. Stimulation with PHA-M enhanced total labeled cells in early pregnancy. In contrast, PHA-M had no such effects in mid-pregnancy[7].
参考文献

[1]D'Costa SS, et al. Phytohemagglutinin inhibits lymphoid tumor growth in vitro and in vivo. Leuk Lymphoma. 2003;44(10):1785-1791.

[2]Merlo JL, et al. Food Restriction Affects Inflammatory Response and Nutritional State in Tuco-tucos (Ctenomys talarum). J Exp Zool A Ecol Genet Physiol. 2016 Dec;325(10):675-687.

[3]Kochubei TO, et al. Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro. Exp Oncol. 2015 Jun;37(2):116-9.

[4]Dupuis G. The binding of phytohemagglutinin M to rat spleen lymphocytes. Quantitative studies. Can J Biochem. 1976;54(8):717‐728

[5]Téllez-Bañuelos MC, González-Ochoa S, Ortiz-Lazareno PC, Rosas-Gonzalez VC, Gómez-Villela J, Haramati J. Low-dose endosulfan inhibits proliferation and induces senescence and pro-inflammatory cytokine production in human lymphocytes, preferentially impacting cytotoxic cells. J Immunotoxicol. 2019;16(1):173‐181

[6]Paßlack N, Kohn B, Doherr MG, Zentek J. Impact of Dietary Protein Concentration and Quality on Immune Function of Cats. PLoS One. 2017;12(1):e0169822. Published 2017 Jan 10

[7]Klein S, Parvizi N. Visualization of immunoreactive growth hormone in cultured peripheral bovine lymphocytes. Growth Horm IGF Res. 2012;22(2):59‐63