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Torin 1

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Chemical Structure| 1222998-36-8 同义名 : -
CAS号 : 1222998-36-8
货号 : A113194
分子式 : C35H28F3N5O2
纯度 : 99%+
分子量 : 607.624
MDL号 : MFCD18782653
存储条件：

粉末 Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 2 mg/mL(3.29 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
靶点

  • mTORC1

    mTORC1, IC50:2 nM

  • mTORC2

    mTORC2, IC50:10 nM

  • mTOR

    mTOR, IC50:4.32 nM
描述 In wild-type MEFs, Torin1 (250 nM) fully arrests proliferation, induces G1/S cell cycle arrest, and reduces cell size more significantly than 50 nM rapamycin[1]. Torin1 exhibits over 800-fold selectivity for mTOR over PI3Kis, demonstrating high specificity compared to other PIKK family kinases, except for DNA-PK[2].
作用机制 Torin 1 is an ATP-competitive inhibitor.
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
697 Growth Inhibition Assay IC50=0.00634 μM SANGER
8-MG-BA Growth Inhibition Assay IC50=0.04629 μM SANGER
A101D Growth Inhibition Assay IC50=0.0265 μM SANGER
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.65mL

0.33mL

0.16mL

8.23mL

1.65mL

0.82mL

16.46mL

3.29mL

1.65mL
参考文献

[1]Thoreen CC, et al, An ATP-competitive mammalian target of rapamycin inhibitor reveals rapamycin-resistant functions of mTORC1. J Biol Chem, 2009, 284(12), 8023-8032.

[2]Liu Q, et al. Discovery of 1-(4-(4-propionylpiperazin-1-yl)-3-(trifluoromethyl)phenyl)-9-(quinolin-3-yl)benzo[h][1,6]naphthyridin-2(1H)-one as a highly potent, selective mammalian target of rapamycin (mTOR) inhibitor for the treatment of cancer. J Med Che