Y-27632

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Chemical Structure| 146986-50-7 同义名 : -
CAS号 : 146986-50-7
货号 : A112358
分子式 : C14H21N3O
纯度 : 99%+
分子量 : 247.336
MDL号 : MFCD03093855
存储条件：

粉末 Keep in dark place,Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 25 mg/mL(101.08 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Y-27632 is a specific inhibitor of Rho-associated kinases[1]. It is usually used for ES cell maintance[2], cell reprogramming[3],improving cell suviving after thawing[4], as well as facilitates cell proliferation and migration[5].
作用机制 Y-27632 can inhibit ROCK1 and ROCK2 in an ATP-competition manner.[1]
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

4.04mL

0.81mL

0.40mL

20.22mL

4.04mL

2.02mL

40.43mL

8.09mL

4.04mL
参考文献

[1]Ishizaki T, Uehata M, et al. Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol. 2000 May;57(5):976-83.

[2]Li X, Meng G, et al. The ROCK inhibitor Y-27632 enhances the survival rate of human embryonic stem cells following cryopreservation. Stem Cells Dev. 2008 Dec;17(6):1079-85.

[3]Zhao Y, Zhao T, et al. A XEN-like State Bridges Somatic Cells to Pluripotency during Chemical Reprogramming. Cell. 2015 Dec 17;163(7):1678-91.

[4]Gauthaman K, Fong CY, et al. ROCK inhibitor Y-27632 increases thaw-survival rates and preserves stemness and differentiation potential of human Wharton's jelly stem cells after cryopreservation. Stem Cell Rev. 2010 Dec;6(4):665-76.

[5]Wang T, Kang W, et al. Rho-kinase inhibitor Y-27632 facilitates the proliferation, migration and pluripotency of human periodontal ligament stem cells. J Cell Mol Med. 2017 Nov;21(11):3100-3112.

[6]Osei-Owusu P, Knutsen RH, et al. Altered reactivity of resistance vasculature contributes to hypertension in elastin insufficiency. Am J Physiol Heart Circ Physiol. 2014;306(5):H654-66.

[7]Mavria G, Vercoulen Y, et al. ERK-MAPK signaling opposes Rho-kinase to promote endothelial cell survival and sprouting during angiogenesis. Cancer Cell. 2006;9(1):33-44.