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Ribavirin

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Chemical Structure| 36791-04-5 同义名 : 利巴韦林 ;Ribasphere;NSC 163039;Ribavirin, Copegus, Rebetol, Virazole, ICN-1229;Tribavirin;RTCA;ICN-1229
CAS号 : 36791-04-5
货号 : A111785
分子式 : C8H12N4O5
纯度 : 98%
分子量 : 244.205
MDL号 : -
存储条件：

粉末 Keep in dark place,Sealed in dry,Room Temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(429.97 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 100 mg/mL(409.49 mM)，配合低频超声助溶
动物实验配方：
生物活性
描述 Hepatitis C virus (HCV) is an enveloped, single-stranded, positive-sense RNA virus in the Flaviviridae family and is the leading cause of cirrhosis and need for liver transplantation. Ribavirin is a nucleoside analogue and broad spectrum antiviral agent that is used with pegylated IFN (Peg-IFN) for HCV treatment. Although ribavirin does not have significant antiviral effect when used alone, it does reduce the number of break-throughs and relapses when used in combination with Peg-IFN. In a mouse model, ribavirin treatment inhibited macrophage inflammatory cytokine response resulting in reduction in alanine aminotransferase (ALT) and histological improvement suggesting ribavirin may act on HCV via an immunomodulatory route. Studies combining IFN-α (used for the treatment of HCV) and ribavirin for 24 or 48 weeks showed increasing proportion of SVR (sustained virologic response) with longer duration and addition of ribavirin suggesting the important role ribavirin plays in improving SVR[3].
作用机制 By depleting endogenous GTP, ribavirin is preferentially incorporated into the growing HCV viral strand leading to reduced viral replication[3].
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
african green monkey Vero cells Function assay 5 days Antiviral activity against RSV A2 infected in african green monkey Vero cells after 5 days by plaque reduction assay, EC50=7 μM 20359898
BHK21 cell line Function assay Inhibition of West Nile virus VLP replicon in BHK21 cell line, EC50=1.1 μM 16539402
E6SM cell lines Function assay Effective concentration required to inhibit Herpes simplex virus-1(HSV-1) / thymine kinase deficient (TK-)B2006 / VMW1837 induced cytopathicity by 50% in E6SM cell lines, EC50=0.005 μM 11495586
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

4.09mL

0.82mL

0.41mL

20.47mL

4.09mL

2.05mL

40.95mL

8.19mL

4.09mL
参考文献

[1]Feld JJ, Hoofnagle JH. Mechanism of action of interferon and ribavirin in treatment of hepatitis C. Nature. 2005 Aug 18;436(7053):967-72.

[2]Crotty S, Maag D, et al.The broad-spectrum antiviral ribonucleoside ribavirin is an RNA virus mutagen. Nat Med. 2000 Dec;6(12):1375-9.

[3]Jain MK, Zoellner C. Role of ribavirin in HCV treatment response: now and in the future. Expert Opin Pharmacother. 2010 Mar;11(4):673-83