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Didox

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Chemical Structure| 69839-83-4 同义名 : NSC-324360
CAS号 : 69839-83-4
货号 : A107593
分子式 : C7H7NO4
纯度 : 98%
分子量 : 169.135
MDL号 : MFCD01667810
存储条件：

粉末 Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(620.81 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
靶点

  • ribonucleotide reductase
描述 Didox is a synthetic antioxidant and a potent ribonucleotide reductase (RNR) inhibitor. Didox treatment of mouse bone marrow-derived mast cells (BMMC) reduced IgE-stimulated degranulation and cytokine production, including IL-6, IL-13, TNF and MIP-1a (CCL3). Furthermore, Didox increased expression of the antioxidant genes superoxide dismutase and catalase, and suppressed DCFH-DA fluorescence, indicating reduced reactive oxygen species production[3]. Didox was active against all human and murine AML (Acute Myeloid Leukemia) lines tested with IC50 values in the low micromolar range (mean IC50 37 µM [range 25.89-52.70 µM]). Didox exposure resulted in DNA damage and p53 induction culminating in apoptosis. Didox was well tolerated and effective against preclinical models of AML[4]. Didox induced caspase-dependent multiple myeloma (MM) cell apoptosis[5]. Nuclear translocation of NF-κβ (p65) in response to LPS is inhibited by didox[6].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

5.91mL

1.18mL

0.59mL

29.56mL

5.91mL

2.96mL

59.12mL

11.82mL

5.91mL
参考文献

[1]Shah KN, Wilson EA, et al. Targeting Ribonucleotide Reductase M2 and NF-κB Activation with Didox to Circumvent Tamoxifen Resistance in Breast Cancer. Mol Cancer Ther. 2015 Nov;14(11):2411-21.

[2]Abdel-Latif GA, Al-Abd AM, et al. The chemomodulatory effects of resveratrol and didox on herceptin cytotoxicity in breast cancer cell lines. Sci Rep. 2015 Jul 9;5:12054.

[3]McLeod JJA, Caslin HL, Spence AJ, Kolawole EM, Qayum AA, Paranjape A, Taruselli M, Haque TT, Kiwanuka KN, Elford HL, Ryan JJ. Didox (3,4-dihydroxybenzohydroxamic acid) suppresses IgE-mediated mast cell activation through attenuation of NFκB and AP-1 transcription. Cell Immunol. 2017 Dec;322:41-48

[4]Cook GJ, Caudell DL, Elford HL, Pardee TS. The efficacy of the ribonucleotide reductase inhibitor Didox in preclinical models of AML. PLoS One. 2014 Nov 17;9(11):e112619

[5]Raje N, Kumar S, Hideshima T, Ishitsuka K, Yasui H, Chhetri S, Vallet S, Vonescu E, Shiraishi N, Kiziltepe T, Elford HL, Munshi NC, Anderson KC. Didox, a ribonucleotide reductase inhibitor, induces apoptosis and inhibits DNA repair in multiple myeloma cells. Br J Haematol. 2006 Oct;135(1):52-61

[6]Matsebatlela TM, Anderson AL, Gallicchio VS, Elford H, Rice CD. 3,4-Dihydroxy-benzohydroxamic acid (Didox) suppresses pro-inflammatory profiles and oxidative stress in TLR4-activated RAW264.7 murine macrophages. Chem Biol Interact. 2015 May 25;233:95-105