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Tectoridin

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Chemical Structure| 611-40-5 同义名 : 射干苷 ;Tectorigenin 7-O-β-D-glucopyranoside;Shekanin
CAS号 : 611-40-5
货号 : A982374
分子式 : C22H22O11
纯度 : 99%+
分子量 : 462.404
MDL号 : MFCD01662715
存储条件：

粉末 Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 250 mg/mL(540.65 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Tectoridin, an isoflavone isolated from Maackia amurensis, is a phytoestrogen and activates estrogen and thyroid hormone receptors. Tectoridin exerts the estrogenic effects via ER-dependent genomic pathway and GPR30-dependent nongenomic pathway[3]. Tectoridin scarcely binds to ER alpha as compared to 17beta-estradiol and genistein. Despite poor binding to ER alpha, tectoridin induced potent estrogenic effects, namely recovery of the population of cells in the S-phase after serum starvation, transactivation of the estrogen response element, and induction of MCF-7 cell proliferation. The tectoridin-induced estrogenic effect was severely abrogated by treatment with U0126, a specific MEK1/2 inhibitor. Tectoridin promoted phosphorylation of ERK1/2, but did not affect phosphorylation of ER alpha at Ser(118). It also increased cellular accumulation of cAMP, a hallmark of GPR30-mediated estrogen signaling[4]. When tectorigenin at a dose of 50 mg/kg was intraperitoneally administered to mice injured by tert-butyl hyperoxide (t-BHP), it significantly inhibited the increase the activities of plasma ALT and AST by 39% and 41%, respectively, in the t-BHP -treated group. The inhibitory effect of tectorigenin is much more potent than that of a commercially available dimethyl diphenyl bicarboxylate. Orally administered tectoridin showed hepatoprotective activity[5]. The radical scavenging activity of tectorigenin protected the viability of Chinese hamster lung fibroblast (V79-4) cells exposed to hydrogen peroxide (H2O2) via activation of extracellular signal regulated kinase (ERK) pathway. Furthermore, tectorigenin reduced the apoptotic cells formation and cell cycle arrest at G2/M phase induced by H2O2. Tectorigenin increased the activities of cellular antioxidant enzymes like superoxide dismutase, catalase, glutathione peroxidase, and also increased their protein level[6].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.16mL

0.43mL

0.22mL

10.81mL

2.16mL

1.08mL

21.63mL

4.33mL

2.16mL
参考文献

[1]Li Y, Wang Q, et al. Fluorescence spectroscopy and docking study in two flavonoids, isolated tectoridin and its aglycone tectorigenin, interacting with human serum albumin: a comparison study. Luminescence. 2016 Feb;31(1):38-46.

[2]Qu J, Wu Z, et al. Excretion of tectoridin metabolites in rat urine and bile orally administrated at different dosages and their inhibitory activity against aldose reductase. Fitoterapia. 2014 Dec;99:99-108.

[3]Shim M, et al. Tectoridin from Maackia amurensis modulates both estrogen and thyroid receptors.Phytomedicine. 2014 Apr 15;21(5):602-6.

[4]Kang K, et al. Tectoridin, a poor ligand of estrogen receptor alpha, exerts its estrogenic effects via an ERK-dependent pathway.Mol Cells. 2009 Mar 31;27(3):351-7.

[5]Hae-Ung Lee,et al. Hepatoprotective effect of tectoridin and tectorigenin on tert-butyl hyperoxide-induced liver injury. J Pharmacol Sci. 2005 Apr;97(4):541-4.

[6]Kyoung Ah Kang,et al. Cytoprotective effect of tectorigenin, a metabolite formed by transformation of tectoridin by intestinal microflora, on oxidative stress induced by hydrogen peroxide, Eur J Pharmacol. 2005 Sep 5;519(1-2):16-23.