产品说明书
TM-25659
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同义名 : | - |
| CAS号 : | 260553-97-7 | |
| 货号 : | A981573 | |
| 分子式 : | C30H28N8 | |
| 纯度 : | 99%+ | |
| 分子量 : | 500.597 | |
| MDL号 : | MFCD32671933 | |
| 存储条件： |
粉末 Inert atmosphere,2-8°C 液体 -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 130 mg/mL(259.69 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO |
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| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | The protein transcriptional co-activator with PDZ-binding motif (TAZ), also referred to as WW domain-containing transcriptional co-regulator (WWTR1), was originally cloned as an interacting protein of the 14-3-3 protein that is involved in the control of cell cycle progression, cell differentiation and apoptosis through interaction with phosphorylated signalling molecules. TM-25659 acts as a TAZ modulator that enhances TAZ expression in the nucleus. Treatment of COS-7 cells with TM-25659 for 30 min increased GFP (green fluorescence protein)-TAZ intensities in a dose-dependent manner. The nuclear level of endogenous TAZ was increased by TM-25659 which was confirmed by immunofluorescence staining with anti-TAZ antibody. In 3T3-L1 cells, TM-25659 treatment for 2 hrs significantly suppressed the normal adipocyte differentiation and lipid droplet formation. As a result of attenuated adipogenic differentiation by TM-25659, the increased levels of PPARγ in differentiated adipocytes were decreased by TM-25659 in a concentration-dependent manner, whereas TAZ expression was substantially elevated. In addition, the adipogenic marker aP2 and adiponectin expression were further decreased by TM-25659. These results demonstrate that TM-25659 acted as a suppressor of PPARγ-dependent adipocyte differentiation. Moreover, the expression levels of osteogenic markers, such as osteocalcin, ALP and osterix were significantly increased by treatment with TM-25659, even at a concentration of 2 µM. In vivo, body weight increase caused by the HFD (high fat diet) was substantially diminished in the TM-25659-treated group compared with the vehicle-treated group[1]. | ||
| 实验方案 | |||
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| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
2.00mL 0.40mL 0.20mL |
9.99mL 2.00mL 1.00mL |
19.98mL 4.00mL 2.00mL |
| 参考文献 |
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