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Bucladesine sodium

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Chemical Structure| 16980-89-5 同义名 : 二丁酰环磷腺苷钠 ;Dibutyryl cAMP sodium salt;DBcAMP sodium salt;DC 2797;Bucladesine (sodium salt);dbcAMP;Bucladesine;Dibutyryl-cAMP
CAS号 : 16980-89-5
货号 : A976872
分子式 : C18H23N5NaO8P
纯度 : 98%
分子量 : 491.368
MDL号 : MFCD00005843
存储条件：

粉末 Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(213.69 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 50 mg/mL(101.76 mM)
动物实验配方：
生物活性
描述 PKA is a ubiquitous cellular kinase, also known as cAMP-dependent protein kinase (cAMP), and it is well-established that plays an important role in regulating several functions of cell processes, including regulation of glycogen, sugar, and lipid metabolism[3]. Dibutyryl-cAMP is a cell-permeable PKA activator that functions by preferentially activating endogenous cAMP. In vitro assays, Dibutyryl-cAMP was found to significantly suppress TNF-a production in a dose-dependent manner, with IC50 values of 247, 28.9, and 25.4 μM, respectively in RAW264.7 cells[4]. Additionally, rat hepatocytes were cultured with cAMP agonists dibutyryl-cAMP inhibits Nitric oxide synthesis and inducible nitric oxide synthase (iNOS) expression through effects on the iNOS promoter region and NF-kB-binding activity[5]. In hepatocytes, Dibutyryl-cAMP can prevent tumor necrosis factor α plus acti-nomycin D (TNFα/ActD)-induced apoptosis via a PKA-dependent mechanism[6]. In vivo model, Dibutyryl-cAMP showed a distinct anti-fibrotic effect via PKA/p-CREB/CBP signaling. Treatment of Dibutyryl-cAMP resulted in the decrease of the number and size of silicosis nodules, inhibition of myofibroblast differentiation, and extracellular matrix deposition[7]. Moreover, 4 days intra-peritoneal injections of bucladesine (600 nM/mouse) significantly reversed zinc chloride- and led acetate-induced avoidance memory retention alterations compared to control animals[8].
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
mouse RAW264.7 cells Function assay 4 h Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced TNFalpha production after 4 hrs by ELISA, IC50=28.9 μM 11000020
mouse S49 cells 500 μM Cytotoxicity assay 20-49 h Cytotoxicity against HGPRTase-deficient mouse S49 cells assessed as growth inhibition at 500 uM after 20 to 49 hrs by time-course study 221658
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.04mL

0.41mL

0.20mL

10.18mL

2.04mL

1.02mL

20.35mL

4.07mL

2.04mL
参考文献

[1]309(3):157-60.

[2]63(9):1205-9.

[3]Dolan S, Nolan AM. Biphasic modulation of nociceptive processing by the cyclic AMP-protein kinase A signalling pathway in sheep spinal cord. Neurosci Lett. 2001 Aug 31;309(3):157-60.

[4]Cho JY, Baik KU, Yoo ES, Yoshikawa K, Park MH. In vitro antiinflammatory effects of neolignan woorenosides from the rhizomes of Coptis japonica. J Nat Prod. 2000 Sep;63(9):1205-9.

[5]Harbrecht BG, Taylor BS, Xu Z, Ramalakshmi S, Ganster RW, Geller DA. cAMP inhibits inducible nitric oxide synthase expression and NF-kappaB-binding activity in cultured rat hepatocytes. J Surg Res. 2001 Aug;99(2):258-64.

[6]Wang Y, Kim PK, Peng X, Loughran P, Vodovotz Y, Zhang B, Billiar TR. Cyclic AMP and cyclic GMP suppress TNFalpha-induced hepatocyte apoptosis by inhibiting FADD up-regulation via a protein kinase A-dependent pathway. Apoptosis. 2006 Mar;11(3):441-51.