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Sodium orthovanadate

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Chemical Structure| 13721-39-6 同义名 : 正钒酸钠 ;NSC 79534
CAS号 : 13721-39-6
货号 : A949923
分子式 : Na3O4V
纯度 : 99%
分子量 : 183.908
MDL号 : MFCD00003511
存储条件：

粉末 Sealed in dry,Room Temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

H2O: 8 mg/mL(43.5 mM)，配合低频超声助溶
动物实验配方：
生物活性
靶点

  • ATPase

    (Na,K)-ATPase, IC50:40 nM
描述 Sodium orthovanadate (SOV) is an inhibitor of protein tyrosine phosphatases, alkaline phosphatases and a number of ATPases, most likely acting as a phosphate analogue[3]. The decreased tyrosine phosphorylation of PTEN and the decreased serine phosphorylation of Akt induced by ischemia were suppressed by SOV, respectively. SOV could alter the phosphorylation status of ASK1 at serine 83 and threonine 845 induced by ischemia[4]. SOV changed SFA (saturated fatty acids) and MUFA (monounsaturated fatty acids) composition in THP-1 macrophages and increased expression of SCD (stearoyl-coenzyme A desaturase). Sodium orthovanadate did not affect the amount of any PUFA[5]. SOV is able to overcome sorafenib resistance and strengthens sorafenib in suppressing sorafenib-resistant HCC (hepatocellular carcinoma) cells in vitro and in animal models. Similar to its action on parental HCC cells, SOV induced cell cycle arrest at G2/M phases by regulating cyclin B1 and cyclin-dependent kinase 1, and apoptosis by reducing mitochondrial membrane potential, in sorafenib-resistant HCC cells[6].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

5.44mL

1.09mL

0.54mL

27.19mL

5.44mL

2.72mL

54.38mL

10.88mL

5.44mL
参考文献

[1]Wu Y, Ou-Yang JP, et al. Hypoglycemic effect of Astragalus polysaccharide and its effect on PTP1B. Acta Pharmacol Sin. 2005 Mar;26(3):345-52.

[2]Cantley LC Jr, Josephson L, et al. Vanadate is a potent (Na,K)-ATPase inhibitor found in ATP derived from muscle. J Biol Chem. 1977 Nov 10;252(21):7421-3.

[3]Gordon JA. Use of vanadate as protein-phosphotyrosine phosphatase inhibitor. Methods Enzymol. 1991;201:477-82

[4]Wu DN, Pei DS, Wang Q, Zhang GY. Down-regulation of PTEN by sodium orthovanadate inhibits ASK1 activation via PI3-K/Akt during cerebral ischemia in rat hippocampus. Neurosci Lett. 2006 Aug 14;404(1-2):98-102

[5]Korbecki J, Gutowska I, Wiercioch M, Łukomska A, Tarnowski M, Drozd A, Barczak K, Chlubek D, Baranowska-Bosiacka I. Sodium Orthovanadate Changes Fatty Acid Composition and Increased Expression of Stearoyl-Coenzyme A Desaturase in THP-1 Macrophages. Biol Trace Elem Res. 2020 Jan;193(1):152-161

[6]Jiang W, Li G, Li W, Wang P, Xiu P, Jiang X, Liu B, Sun X, Jiang H. Sodium orthovanadate overcomes sorafenib resistance of hepatocellular carcinoma cells by inhibiting Na+/K+-ATPase activity and hypoxia-inducible pathways. Sci Rep. 2018 Jun 26;8(1):9706