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3'-Azido-3'-deoxythymidine

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Chemical Structure| 30516-87-1 同义名 : 叠氮胸苷 ;Zidovudine;Azidothymidine;brand name Retrovir.;BW A509U;NSC 602670;ZDV;AZT
CAS号 : 30516-87-1
货号 : A944627
分子式 : C10H13N5O4
纯度 : 99%
分子量 : 267.241
MDL号 : MFCD00006536
存储条件：

粉末 Keep in dark place,Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(392.9 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 15 mg/mL(56.13 mM)，配合低频超声，并水浴加热至45℃助溶
动物实验配方：
生物活性
描述 Zidovudine is a nucleoside reverse transcriptase inhibitor (NRTI), widely used to treat HIV infection. It acts by interfering with viral reverse transcriptase, thereby inhibiting human immunodeficiency virus (HIV) replication. Zidovudine inhibits SVG, Primary human fetal astrocytes (PFA), peripheral blood mononuclear cells (PBMC), and monocyte-derived macrophages (MDM) with EC50 of 17, 1311, 8, and 5 nM, respectively. Zidovudine inhibits SVG, PFA, PBMC, and MDM with EC90 of 0.205 μM, 44.157 μM, 0.481 μM, and 0.219 μM, respectively[3]. Zidovudine reduces the incidence of AIDS dementia complex (ADC) and appears to prolong survival in these patients, and improves other neurological complications of HIV infection[4]. In acquired immunodeficiency syndrome (AIDS) and AIDS-related complex (ARC) patients, orally and intravenously administered zidovudine is effective in reducing the incidence of opportunistic infections and neoplasms, increasing helper T lymphocyte numbers, and improving survival rates and quality of life[5].
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
AA5 cells Function assay Antiviral activity against of Human immunodeficiency virus 1 3B infected in AA5 cells infected with 1.56 uL of virus stock assessed as inhibition of viral Reverse transcriptase by [3H]TTP incorporation assay, IC50=0.3 nM 20086149
C3H/3T3 cells Function assay Concentration of compound required to inhibit HIV-1 induced cytopathogenicity of MSV-induced transformation of C3H/3T3 cells by 50%, EC50=0.02 μM 2016718
C8166 cells Function assay Inhibition of HIV1-3B replication in C8166 cells, EC50=1.9 nM 16279773
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.74mL

0.75mL

0.37mL

18.71mL

3.74mL

1.87mL

37.42mL

7.48mL

3.74mL
参考文献

[1]Panther LA, Coombs RW, et al. Unintegrated HIV-1 circular 2-LTR proviral DNA as a marker of recently infected cells: relative effect of recombinant CD4, zidovudine, and saquinavir in vitro. J Med Virol. 1999 Jun;58(2):165-73.

[2]Chiang G, Sassaroli M, et al. Inhibition of HIV-1 replication by hydroxychloroquine: mechanism of action and comparison with zidovudine. Clin Ther. 1996 Nov-Dec;18(6):1080-92.

[3]Rachlis A, Fanning MM. Zidovudine toxicity. Clinical features and management. Drug Saf. 1993 Apr;8(4):312-20

[4]Wilde MI, Langtry HD. Zidovudine. An update of its pharmacodynamic and pharmacokinetic properties, and therapeutic efficacy. Drugs. 1993 Sep;46(3):515-578

[5]Langtry HD, Campoli-Richards DM. Zidovudine. A review of its pharmacodynamic and pharmacokinetic properties, and therapeutic efficacy. Drugs. 1989 Apr;37(4):408-50