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Isoorientin

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Chemical Structure| 4261-42-1 同义名 : 异荭草苷 ;Homoorientin;Lespecapitioside;Lutonaretin;Luteolin-6-C-glucoside
CAS号 : 4261-42-1
货号 : A844426
分子式 : C21H20O11
纯度 : 99%+
分子量 : 448.377
MDL号 : MFCD00017433
存储条件：

粉末 Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(234.18 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Isoorientin (or homoorientin) is a flavone, which is a chemical flavonoid-like compound, and a 6-C-glucoside of luteolin. In PC (pancreatic cancer), isoorientin strongly inhibited the survival of the cells, induced cell apoptosis, and decreased its malignancy by reversing the expression of epithelial-mesenchymal transition and matrix metalloproteinase and decreased vascular endothelial growth factor expression[3]. Isoorientin was found to be a potent inhibitor of COX-2with an IC50 value of 39 μM[4]. Isoorientin reduced inflammation in RAW 264.7 cell line in vitro and carrageenan induced inflammatory animal model systems in vivo. Isoorientin, a selective inhibitor of COX-2, not only exerts anti-inflammatory effects in LPS induced RAW cells and carrageenan induced inflammatory model systems but also exhibits potent antioxidant properties[5]. Isoorientin can inhibit GSK3β by increasing p-GSK3β and regulate the downstream signal molecules to inhibit inflammation and protect the integrity of the blood-brain barrier and the homeostasis in the brain[6].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.23mL

0.45mL

0.22mL

11.15mL

2.23mL

1.12mL

22.30mL

4.46mL

2.23mL
参考文献

[1]Bouaziz-Ketata H, Zouari N, et al. Flavonoid profile and antioxidant activities of methanolic extract of Hyparrhenia hirta (L.) Stapf. Indian J Exp Biol. 2015 Apr;53(4):208-15.

[2]Cao J, Xia X, et al. Flavonoids profiles, antioxidant, acetylcholinesterase inhibition activities of extract from Dryoathyrium boryanum (Willd.) Ching. Food Chem Toxicol. 2013 May;55:121-8.

[3]Ye T, Su J, Huang C, Yu D, Dai S, Huang X, Chen B, Zhou M. Isoorientin induces apoptosis, decreases invasiveness, and downregulates VEGF secretion by activating AMPK signaling in pancreatic cancer cells. Onco Targets Ther. 2016 Dec 12;9:7481-7492

[4]Sumalatha M, Munikishore R, Rammohan A, Gunasekar D, Kumar KA, Reddy KK, Azad R, Reddanna P, Bodo B. Isoorientin, a Selective Inhibitor of Cyclooxygenase-2 (COX-2) from the Tubers of Pueraria tuberosa. Nat Prod Commun. 2015 Oct;10(10):1703-4

[5]Anilkumar K, Reddy GV, Azad R, Yarla NS, Dharmapuri G, Srivastava A, Kamal MA, Pallu R. Evaluation of Anti-Inflammatory Properties of Isoorientin Isolated from Tubers of Pueraria tuberosa. Oxid Med Cell Longev. 2017;2017:5498054

[6]Li Y, Zhao Y, Tan X, Liu J, Zhi Y, Yi L, Bai S, Du Q, Li QX, Dong Y. Isoorientin Inhibits Inflammation in Macrophages and Endotoxemia Mice by Regulating Glycogen Synthase Kinase 3β. Mediators Inflamm. 2020 Oct 27;2020:8704146