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Crustecdysone

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Chemical Structure| 5289-74-7 同义名 : β-蜕皮甾酮 ;20-Hydroxyecdysone;Ecdysterone;Isoinokosterone;Commisterone;B-ecdysone;20E
CAS号 : 5289-74-7
货号 : A844114
分子式 : C27H44O7
纯度 : 95%
分子量 : 480.634
MDL号 : MFCD00036740
存储条件：

粉末 Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(218.46 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 8 mg/mL(16.64 mM)，配合低频超声，并水浴加热至45℃助溶
动物实验配方：

1% DMSO+30% polyethylene glycol+1% Tween 80+water 30 mg/mL suspension

生物活性
描述 20-Hydroxyecdystone (20E) is an ecdysteroid hormone which controls molting and reproduction in arthropods. 20E also produces a variety of effects in vertebrates, including enhancing protein synthesis and skeletal muscle regeneration. 20E treatment tended to attenuate disuse muscle atrophy and reduced ubiquitination only in soleus muscle[3]. The progressive increase in systolic blood pressure with age in SHR (spontaneously hypertensive rats) rats was significantly lower in animals treated with either 5 or 10mg/kg body weight of 20-HE (20-hydroxyecdysone). Echocardiography after six weeks of treatment demonstrated that the left ventricular chamber of SHR rats treated with 20-HE was smaller than that of SHR controls, while contractility was not affected by 20-HE. Histological images also demonstrated a decrease in cardiomyocyte cross-sectional area in 20-HE treated groups. Interestingly, treatment with 20-HE caused a shift in cardiac myosin heavy chain towards more β-isoforms. SHR rats treated with 20-HE also exhibited a decrease in seminal vesicular weight and an increase in testicular weight, especially at a dose of 10mg/kg body weight. 20-HE has a beneficial effect on reducing blood pressure and consequently preventing dilated cardiac hypertrophy in SHR rats[4]. Exposure of daphnids to elevated concentrations of 20-hydroxyecdysone caused premature and incomplete ecdysis resulting in death. Tributyltin exaggerated this effect of exogenous 20-hydroxyecdysone. Further, exposure of daphnids to tributyltin enhanced the inductive effects of 20-hydroxyecdysone on expression of the 20-hydroxyecdysone-inducible gene HR3[5].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.08mL

0.42mL

0.21mL

10.40mL

2.08mL

1.04mL

20.81mL

4.16mL

2.08mL
参考文献

[1]Hu J, Zhao TZ, et al. Protective effects of 20-hydroxyecdysone on CoCl(2)-induced cell injury in PC12 cells. J Cell Biochem. 2010 Dec 15;111(6):1512-21.

[2]Kizelsztein P, Govorko D, et al. 20-Hydroxyecdysone decreases weight and hyperglycemia in a diet-induced obesity mice model. Am J Physiol Endocrinol Metab. 2009 Mar;296(3):E433-9.

[3]Hirunsai M, Yimlamai T, Suksamrarn A. Effect of 20-Hydroxyecdysone on Proteolytic Regulation in Skeletal Muscle Atrophy. In Vivo. 2016;30(6):869-877

[4]Phungphong S, Kijtawornrat A, Chaiduang S, Saengsirisuwan V, Bupha-Intr T. 20-Hydroxyecdysone attenuates cardiac remodeling in spontaneously hypertensive rats. Steroids. 2017;126:79-84

[5]Wang YH, Kwon G, Li H, Leblanc GA. Tributyltin synergizes with 20-hydroxyecdysone to produce endocrine toxicity. Toxicol Sci. 2011;123(1):71-79