产品说明书

AZ20

Print
Chemical Structure| 1233339-22-4 同义名 : -
CAS号 : 1233339-22-4
货号 : A838709
分子式 : C21H24N4O3S
纯度 : 99%+
分子量 : 412.505
MDL号 : MFCD25976730
存储条件：

粉末 Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(254.54 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
靶点

  • ATR

    ATR, IC50:5 nM
描述 AZ20 is a potent inhibitor of the ATR kinase, effectively inhibiting ATR extracted from HeLa nuclear extracts and ATR-mediated phosphorylation of Chk1 in HT29 colorectal adenocarcinoma cells with IC50 values of 5 nM and 50 nM, respectively[1].
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
HT29 cells Function assay 1 h  Inhibition of ATR-mediated CHK1 phosphorylation at serine 345 in human HT29 cells after 1 hr in presence of 4-nitroquinoline 1-oxide, IC50=0.05 μM 23394205
human MDA-MB-468 cells Function assay Inhibition of mTOR-mediated AKT phosphorylation at serine 473 in human MDA-MB-468 cells, IC50=2.4 μM 23394205
LoVo cells Growth inhibition assay 72 h Growth inhibition of human LoVo cells after 72 hrs by MTS assay, GI50=0.2 μM 23394205
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.42mL

0.48mL

0.24mL

12.12mL

2.42mL

1.21mL

24.24mL

4.85mL

2.42mL
参考文献

[1]Foote KM, et al. Discovery of 4-{4-[(3R)-3-Methylmorpholin-4-yl]-6-[1-(methylsulfonyl)cyclopropyl]pyrimidin-2-yl}-1H-indole (AZ20): a potent and selective inhibitor of ATR protein kinase with monotherapy in vivo antitumor activity. J Med Chem. 2013 Mar 14