生物活性 | |||
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描述 | Interleukin-1(IL-1) is a pro-inflammatory cytokine that plays a role in many acute and chronic human diseases, and elevated levels of IL-1 can be measured in human tissue or body fluids from patient with rheumatoid arthritis (RA), osteoarthritis, chronic obstructive pulmonary disease, asthma, inflammatory bowel and other diseases[1]. CP 424174 is identified as potent inhibitor of stimulus-coupled IL-1β post-translational processing. It blocks the formation of mature IL-1β without increasing the amount of procytokine. It inhibits ATP-induced proteolytic cleavage of pro-IL-1β, measured by secretion of the 17 kDa mature form of IL-1β, in LPS-primed human blood-derived monocytes when used at concentrations of 0.5 and 2.5 µM. CP 424,174 inhibits ATP-induced IL-1α and IL-1β production in LPS-primed mouse peritoneal macrophages (ED50s = 15 and 14 mg/kg, respectively)[2]. |
实验方案 | |||
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1mg | 5mg | 10mg | |
1 mM 5 mM 10 mM |
2.21mL 0.44mL 0.22mL |
11.04mL 2.21mL 1.10mL |
22.08mL 4.42mL 2.21mL |
参考文献 |
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