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Nile Red

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Chemical Structure| 7385-67-3 同义名 : Nile Blue A oxazone;Phenoxazone 9
CAS号 : 7385-67-3
货号 : A819053
分子式 : C20H18N2O2
纯度 : 99%+
分子量 : 318.369
MDL号 : MFCD00011639
存储条件：

粉末 Keep in dark place,Sealed in dry,Room Temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 2 mg/mL(6.28 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

无水乙醇: 1 mg/mL(3.14 mM)，配合低频超声，并水浴加热至45℃助溶，注意：无水乙醇开封后，易挥发，也会吸收空气中的水分，导致溶解能力下降，请避免使用开封较久的乙醇
动物实验配方：
生物活性
描述 Nile red (Nile blue oxazone) is a lipophilic dye with environment-sensitive fluorescence properties, exhibiting strong fluorescence in lipid-rich environments and minimal fluorescence in aqueous media. It is particularly effective for detecting intracellular lipid droplets via fluorescence microscopy and flow cytometry[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.14mL

0.63mL

0.31mL

15.71mL

3.14mL

1.57mL

31.41mL

6.28mL

3.14mL
参考文献

[1]Greenspan P, et al. Nile red: a selective fluorescent stain for intracellular lipid droplets. J Cell Biol. 1985 Mar;100(3):965-73.

[2]Wilber Escorcia, et al. Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining. J Vis Exp. 2018 Mar 5;(133):57352.