MDK83190

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Chemical Structure| 79183-19-0 同义名 : Apoptosis Activator 2;N-(3,4-dichlorobenzyl) Isatin;N-(3,4-dichlorobenzyl) Isatin. MDK83190;AAII
CAS号 : 79183-19-0
货号 : A804232
分子式 : C15H9Cl2NO2
纯度 : 99%+
分子量 : 306.144
MDL号 : MFCD00141556
存储条件：

Pure form Sealed in dry,Room Temperature

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 45 mg/mL(146.99 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Apoptosis is an evolutionarily conserved process that regulates development and homeostasis, and defects in this pathway are implicated in both tumorigenesis and multidrug resistance. Apoptosis activator 2 is a potent apoptosis Inducer in cells, strongly inducing caspase-3 activation, PARP cleavage, and DNA fragmentation, and finally killing cells with an IC50 of ≈4 μM. Of the five normal cell lines tested, apoptosis activator 2 had no effect except at the highest concentration (50 μM) when tested against HMEC, PREC, or MCF-10A cells. Apoptosis activator 2 exerted a cytostatic effect on the majority of tumor cell lines tested, inhibiting cell growth by 50–100% at 10 μM in 40 of 48 tumor cell lines tested[3]. TUNEL test in gastric adenocarcinoma cell line (AGS) cells treated with 10 μM of apoptosis activator 2 showed a significant increase in apoptosis in comparison with non-treated AGS cells[4].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.27mL

0.65mL

0.33mL

16.33mL

3.27mL

1.63mL

32.66mL

6.53mL

3.27mL
参考文献

[1]Alavian KN, Sgadò P, et al. Elevated P75NTR expression causes death of engrailed-deficient midbrain dopaminergic neurons by Erk1/2 suppression. Neural Dev. 2009 Mar 16;4:11.

[2]Nguyen JT, Wells JA. Direct activation of the apoptosis machinery as a mechanism to target cancer cells. Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7533-8. Epub 2003 Jun 13.

[3]Nguyen JT, Wells JA. Direct activation of the apoptosis machinery as a mechanism to target cancer cells. Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7533-8. doi: 10.1073/pnas.1031631100. Epub 2003 Jun 13. PMID: 12808146; PMCID: PMC164621.

[4]Farivar TN, Najafipour R, Johari P. Nano - drug Delivery of Apoptosis Activator 2 to AGS Cells by Liposomes Conjugated with Anti-TROP2 Antibody. N Am J Med Sci. 2012 Nov;4(11):582-5. doi: 10.4103/1947-2714.103319. PMID: 23181231; PMCID: PMC3503378.