产品说明书
Propidium Iodide
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同义名 : | 碘化丙啶 ;PI |
| CAS号 : | 25535-16-4 | |
| 货号 : | A795950 | |
| 分子式 : | C27H34I2N4 | |
| 纯度 : | 95% | |
| 分子量 : | 668.395 | |
| MDL号 : | MFCD00011921 | |
| 存储条件： |
粉末 Keep in dark place,Sealed in dry,2-8°C 液体 -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 105 mg/mL(157.09 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO H2O: 3 mg/mL(4.49 mM)，配合低频超声，并水浴加热至45℃助溶 |
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| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | Propidium iodide (PI) is a red-fluorescent nuclear and chromosome counterstain which can bind to double stranded DNA by intercalating between base pairs. It is a cell impermeant dye, which can be excluded from viable cells. Thus it is widely used for the evaluation of apoptosis in different experimental models. Propidium iodide is excited at 488 nm and, with a relatively large Stokes shift, emits at a maximum wavelength of 617 nm. General protocol: 1. Trypsinize and harvest cells in the appropriate manner and wash in PBS. 2. Fix cells into 0.5 ml 70% EtOH for at least 1h. 70% EtOH should be pre-cooled to -20 °C overnight. PAUSE POINT: Cells can be stored in ethanol solution at –20°C for several weeks. 3. Spin down cells for 2 min at 2,000 rpm. 4. Discard the supernatant. Be careful to avoid cell loss. 5. Wash in PBS for two times as: resuspend cell pellet in 0.5mL 1xPBS, spin down cells for 2 min at 2,000 rpm, discard the supernatant and repeat these steps. 6. Resuspend cell pellet in 0.5 ml PBS containing 10 μg/ml RNase A and 20 μg/ml PI stock solution, transfer to FACS tubes. Note: The addition of RNase A could ensure that only DNA is stained. 7. Incubate at room temperature in the dark for 30 min. 8. Analyze cells by flow cytometry. Use 488-nm laser line for excitation. Measure red fluorescence (>600 nm) and side scatter. Optimal protocol: Fluorochrome solution: 0.1% sodium citrate (wt/v), 0.1% Triton X-100 |
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| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
|
1 mM 5 mM 10 mM |
1.50mL 0.30mL 0.15mL |
7.48mL 1.50mL 0.75mL |
14.96mL 2.99mL 1.50mL |
