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Baccatin III

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Chemical Structure| 27548-93-2 同义名 : 巴卡丁 III ;NSC 330753;Baccatin Ⅲ
CAS号 : 27548-93-2
货号 : A777329
分子式 : C31H38O11
纯度 : 98%
分子量 : 586.627
MDL号 : MFCD00153921
存储条件：

粉末 Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 35 mg/mL(59.66 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Baccatin III is a precursor to the anti-cancer drug paclitaxel, it's a natural product isolated and purified from the barks of Taxus chinensis. Baccatin III, which is the precursor for the semisynthesis of paclitaxel, exerts anti-tumor immunomodulatory activity in very low doses (0.05-0.5mg/kg). Oral administration of baccatin III significantly reduced the growth of tumors induced by engrafting BALB/c mice with either 4 T1 mammary carcinoma or CT26 colon cancer cells. Baccatin III (0.5mg/kg) did not exert anti-tumor activity in athymic nude mice. Baccatin III decreased the accumulation of MDSCs (Myeloid-derived suppressor cells) in the spleens of the tumor-bearing mice[3]. Baccatin III inhibited cell proliferation of a number of cancer cell lines with IC50 ranging from 2 to 5 μM for fungal baccatin III. It also induced apoptosis in JR4-Jurkat cells with a possible involvement of anti-apoptotic Bcl2 and loss in mitochondrial membrane potential, and was unaffected by inhibitors of caspase-9,-2 or -3 but was prevented in presence of caspase-10 inhibitor. DNA fragmentation was also observed in cells treated with baccatin III[4].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.70mL

0.34mL

0.17mL

8.52mL

1.70mL

0.85mL

17.05mL

3.41mL

1.70mL
参考文献

[1]Gallego A, Imseng N, et al. Development of a hazel cell culture-based paclitaxel and baccatin III production process on a benchtop scale. J Biotechnol. 2015 Feb 10;195:93-102.

[2]Li Y, Yang J, et al. Isolation and identification of a 10-deacetyl baccatin-III-producing endophyte from Taxus wallichiana. Appl Biochem Biotechnol. 2015 Feb;175(4):2224-31.

[3]Lee YH, Lee YR, Park CS, Im SA, Song S, Hong JT, Whang BY, Kim K, Lee CK. Baccatin III, a precursor for the semisynthesis of paclitaxel, inhibits the accumulation and suppressive activity of myeloid-derived suppressor cells in tumor-bearing mice. Int Immunopharmacol. 2014 Aug;21(2):487-93

[4]Chakravarthi BV, Sujay R, Kuriakose GC, Karande AA, Jayabaskaran C. Inhibition of cancer cell proliferation and apoptosis-inducing activity of fungal taxol and its precursor baccatin III purified from endophytic Fusarium solani. Cancer Cell Int. 2013 Oct 23;13(1):105