Compstatin
产品说明书
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同义名 : | - |
| CAS号 : | 206645-99-0 | |
| 货号 : | A773831 | |
| 分子式 : | C66H99N23O17S2 | |
| 纯度 : | 99%+ | |
| 分子量 : | 1550.766 | |
| MDL号 : | MFCD09263618 | |
| 存储条件： |
Pure form Keep in dark place, inert atmosphere, store in freezer, under -20°C In solvent -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
H2O: 100 mg/mL(64.48 mM)，配合低频超声助溶 |
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| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | The complement system is a key part of the innate and adaptive immune system and plays a major role in homeostasis by clearing altered host cells and invading pathogens. Compstatin, a 13-residue peptide, is an effective inhibitor of the activation of complement component C3 with IC50 value of 12 μM and thus blocks a central and crucial step in the complement cascade[1]. In vitro studies of compstatin in human blood indicated that a major pathway of biotransformation was the removal of Ile1, which could be blocked by N-acetylation of the peptide[2]. Subsequent interaction analysis confirmed that compstatin binds NHP C3 (baboon) and human C3 with similar affinity, but it does not bind to mouse or rat C3[3]. Compstatin produced complete inhibition at a total dose of 21 mg/kg when given as a combination of bolus injection and infusion. It completely inhibited in vivo heparin/protamine-induced complement activation without adverse effects on heart rate or systemic arterial, central venous, and pulmonary arterial pressures[4]. | ||
| 作用机制 | The crystal structure reveals that the compstatin-binding site is formed by the macroglobulin (MG) domains 4 and 5[1]. | ||
| 细胞研究 | |||||
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| 细胞系 | 浓度 | 检测类型 | 检测时间 | 活性说明 | 数据源 |
| RPE cells | 50 uM | Function assay | Inhibition of complement activation in human fetal RPE cells assessed as reduction in C5b-9 formation at 50 uM by fluorescence assay in presence of complement-competant human serum | 25494040 | |
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
0.64mL 0.13mL 0.06mL |
3.22mL 0.64mL 0.32mL |
6.45mL 1.29mL 0.64mL |
| 参考文献 |
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