MKC9989

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Chemical Structure| 1338934-20-5 同义名 : -
CAS号 : 1338934-20-5
货号 : A724612
分子式 : C17H20O7
纯度 : 99%+
分子量 : 336.337
MDL号 : MFCD28386191
存储条件：

Pure form Inert atmosphere,Store in freezer, under -20°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 50 mg/mL(148.66 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 At a concentration of 10 μM, MKC9989 completely blocks both basal and thapsigargin-induced splicing of XBP1 mRNA. These effects persist even in cells pre-treated with thapsigargin, suggesting MKC9989's ability to fully reverse XBP1 splicing once the UPR is triggered. Additionally, MKC9989 significantly enhances the stability of the RIDD target CD59 mRNA when co-administered with thapsigargin compared to thapsigargin treatment alone. It also slightly elevates CD59 mRNA levels in unstressed cells, likely due to inhibiting baseline RIDD activity. Unlike its impact on XBP1 splicing, MKC9989 moderately increases CD59 levels when administered 2 hours after thapsigargin treatment. Lastly, MKC9989's effectiveness against XBP1 mRNA splicing (EC50=0.33 μM) is comparable to its potency against RNA cleavage in vitro[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.97mL

0.59mL

0.30mL

14.87mL

2.97mL

1.49mL

29.73mL

5.95mL

2.97mL
参考文献

[1]Sanches M, et al. Structure and mechanism of action of the hydroxy-aryl-aldehyde class of IRE1 endoribonuclease inhibitors. Nat Commun. 2014 Aug 28;5:4202.