产品说明书

HAMNO

Print
Chemical Structure| 138736-73-9 同义名 : NSC111847
CAS号 : 138736-73-9
货号 : A724552
分子式 : C17H13NO2
纯度 : 99%+
分子量 : 263.291
MDL号 : MFCD28411834
存储条件：

粉末 Keep in dark place,Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 50 mg/mL(189.9 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Replication protein A (RPA), a major eukaryotic ssDNA-binding protein, is essential for all metabolic processes that involve ssDNA, including DNA replication, repair, and damage signaling[1]. During DNA replication stress, RPA binds the ssDNA exposed downstream of stalled primer/template (P/T) junctions, releasing Rad6/Rad18. RPA restricted the resident PCNAs to the upstream duplex regions by physically blocking diffusion of PCNA along ssDNA, and this activity was required for efficient monoubiquitination of PCNA on DNA[2]. HAMNO is a potent and selective inhibitor of replication protein A (RPA) interactions with proteins involved in the replication stress response. HAMNO was first identified as a RPA DBD-F inhibitor. It affects RPA and ATR phosphorylation directly by inhibiting DBD-F interactions with replication stress response protein involved in activating ATR. Ser22 of RPA showed highly phosphorylated after 2 h of treatment with 20μM of etoposide, which is reduced with the addition of 2 μM HAMNO. Cancer derived UMSCC38 cells, as well as another cancer cell line, UMSCC11B, have prominent γ-H2AX staining, particularly after incubation with 20 μM HAMNO. Both UMSCC38 and OKF4 cells present increased γ-H2AX staining after addition of HAMNO, with the greatest increase in signal occurring in S-phase[3].The experiment of Fanconi anemia (FA) repair pathway, which is induced by RPA, showed that HAMNO significantly induced FANCD2 monoubiquitination and foci formation and also caused increased level of γ-H2AX and S-phase accumulation in FA-deficient cells[4].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.80mL

0.76mL

0.38mL

18.99mL

3.80mL

1.90mL

37.98mL

7.60mL

3.80mL
参考文献

[1]Olga M Mazina,et al. Replication protein A binds RNA and promotes R-loop formation. J Biol Chem. 2020. 295(41), 14203-14213.

[2]Mark Hedglin,et al. Replication protein A dynamically regulates monoubiquitination of proliferating cell nuclear antigen. J Biol Chem. 2019. 294(13), 5157-5168.

[3]Glanzer JG, et al. RPA inhibition increases replication stress and suppresses tumor growth. Cancer Res. 2014. 74(18), 5165-72.

[4]Jang, S., Kim, J. M. The RPA inhibitor HAMNO sensitizes Fanconi anemia pathway-deficient cells. Cell cycle. 2022. 21(14), 1468-1478.