Pyrvinium pamoate

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Chemical Structure| 3546-41-6 同义名 : 扑蛲灵;吡维氯铵;吡维铵;恩波酸吡维铵 ;Pyrvinium embonate;Pyrvinium (pamoate);NSC 223622
CAS号 : 3546-41-6
货号 : A722097
分子式 : C75H70N6O6
纯度 : 99%
分子量 : 1151.395
MDL号 : MFCD00010090
存储条件：

Pure form Keep in dark place,Inert atmosphere,Room temperature

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 16 mg/mL(13.9 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Pyrvinium Pamoate (PP), an anthelmintic drug, inhibited proliferation of different subtypes of breast cancer cells (luminal: MCF-7, claudin-low: MDA-MB-231, basal-like: MDA-MB-468 and Her-2 enriched: SkBr-3) as a novel WNT pathway inhibitor. In the xenograft model, Pyrvinium pamoate (500 nM)-pretreatment strongly delays tumor size and tumor weight, and the tumor volume is markedly decreased. Besides, the metastatic potential and expression of EMT markers (such as N-cadherin, vimentin, Snail) were also found suppressed by PP[1]. PP blocked colon cancer cell growth in vitro in a dose-dependent manner with great differences in the inhibitory concentration (IC50), ranging from 0.6 × 10-6 to 65 × 10-6 M for colon cancer cells with mutations in WNT signaling. In addition, PP demonstrated a cytotoxic effect on primary colon cancer samples[2]. Pyrvinium pamoate imposes specific toxicity on cardiac fibroblasts in ischemia (IC50=9.5 nM). The cytotoxic effect of Pyrvinium pamoate on cardiac fibroblasts specifically under glucose- and glutamine-deficient condition[3]. Pyrvinium pamoate remarkably inhibited cell viability and colony formation ability. Treatment with pyrvinium pamoate induced intrinsic pathway-dependent apoptosis accompanied with a decline of anti-apoptotic XIAP and Survivin, and an overt increase of pro-apoptotic Bax. In addition, pyrvinium pamoate significantly inhibited the migration and invasion in vitro[4].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

0.87mL

0.17mL

0.09mL

4.34mL

0.87mL

0.43mL

8.69mL

1.74mL

0.87mL
参考文献

[1]Xu L, Zhang L, Hu C, et al. WNT pathway inhibitor pyrvinium pamoate inhibits the self-renewal and metastasis of breast cancer stem cells. Int J Oncol. 2016;48(3):1175-1186

[2]Wiegering A, Uthe FW, Hüttenrauch M, et al. The impact of pyrvinium pamoate on colon cancer cell viability. Int J Colorectal Dis. 2014;29(10):1189-1198

[3]Murakoshi M, Saiki K, Urayama K, Sato TN. An anthelmintic drug, pyrvinium pamoate, thwarts fibrosis and ameliorates myocardial contractile dysfunction in a mouse model of myocardial infarction. PLoS One. 2013;8(11):e79374.

[4]Zheng L, Liu Y, Pan J. Inhibitory effect of pyrvinium pamoate on uveal melanoma cells involves blocking of Wnt/β-catenin pathway. Acta Biochim Biophys Sin (Shanghai). 2017;49(10):890-898