产品说明书
Homoharringtonine
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同义名 : | Omacetaxine mepesuccinate;HHT;homoharringtonine, 3H-labeled, (3(R))-isomer;Ceflatonin;NSC 141633;Myelostat;CGX-635 |
| CAS号 : | 26833-87-4 | |
| 货号 : | A718170 | |
| 分子式 : | C29H39NO9 | |
| 纯度 : | 98% | |
| 分子量 : | 545.621 | |
| MDL号 : | MFCD05618221 | |
| 存储条件： |
粉末 Sealed in dry,2-8°C 液体 -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 50 mg/mL(91.64 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO H2O: 1 mg/mL(1.83 mM)，配合低频超声助溶 |
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| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | Homoharringtonine effectively suppresses IL-6-induced STAT3 phosphorylation in a dose- and time-dependent manner. Moreover, it impedes cellular growth, viability, and colony formation while promoting apoptosis via the mitochondrial pathway. We evaluate its cytotoxicity on human NSCLC cell lines A549 (wild type EGFR) and NCI-H1975 (H1975, mutant EGFR with L858R and T790M), using Gefitinib as a control. Through MTT assays, we find that Homoharringtonine demonstrates moderate cytotoxicity towards A549 cells, with an IC50 of 3.7 μM, while H1975 cells exhibit heightened sensitivity, with an IC50 of 0.7 μM. Furthermore, Homoharringtonine suppresses the proliferation and growth of both A549 and H1975 cells in a dose- and time-dependent manner, as confirmed by MTT assays. Additionally, using trypan blue exclusion assays, we observe a rapid reduction in viable A549 and H1975 cells in response to Homoharringtonine treatment, again in a dose- and time-dependent manner. Notably, Homoharringtonine significantly curtails the clonogenic potential of both A549 and H1975 cells[1]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
1.83mL 0.37mL 0.18mL |
9.16mL 1.83mL 0.92mL |
18.33mL 3.67mL 1.83mL |
| 参考文献 |
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