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NIH-12848

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Chemical Structure| 959551-10-1 同义名 : -
CAS号 : 959551-10-1
货号 : A709364
分子式 : C20H14F3N3S
纯度 : 99%+
分子量 : 385.406
MDL号 : MFCD30738011
存储条件：

粉末 Keep in dark place,Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(272.44 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 NIH-12848 inhibits PI5P4Kγ with an IC50 of approximately 1 μM, yet does not inhibit the α and β isoforms of PI5P4K even at concentrations up to 100 μM. NIH-12848 can suppress the translocation of Na+/K+-ATPase to the plasma membrane that occurs when mpkCCD cells grow to confluence and can reversibly prevent the formation of "domes" on the culture dish. Both effects induced by NIH-12848 can be mimicked by specific RNAi knockdown of PI5P4Kγ, but not by knockdown of PI5P4Ks α or β. NIH-12848 may serve as a potent tool for exploring the cell physiology of PI5P4Ks[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.59mL

0.52mL

0.26mL

12.97mL

2.59mL

1.30mL

25.95mL

5.19mL

2.59mL
参考文献

[1]Clarke JH, et al. The function of phosphatidylinositol 5-phosphate 4-kinase γ (PI5P4Kγ) explored using a specific inhibitor that targets the PI5P-binding site. Biochem J. 2015 Mar 1;466(2):359-67.