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JK184

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Chemical Structure| 315703-52-7 同义名 : -
CAS号 : 315703-52-7
货号 : A706302
分子式 : C19H18N4OS
纯度 : 95%
分子量 : 350.437
MDL号 : MFCD01044465
存储条件：

粉末 Keep in dark place,Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 50 mg/mL(142.68 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 JK184 is formulated to antagonize Hedgehog (Hh) signaling by impeding glioma (Gli)-dependent transcriptional activity in a dose-dependent manner. It significantly inhibits the proliferation of human umbilical vein endothelial cells (HUVECs) with an IC50 of 6.3 μg/mL after three days of incubation. To assess the anti-tumor effect of JK184, an MTT assay is conducted in Panc-1 and BxPC-3 cells after treatment with specified compound concentrations, revealing a half-maximal inhibitory concentration (IC50) of JK184 (23.7 ng/mL in Panc-1 and 34.3 ng/mL in BxPC-3)[1]. Claudin-low cell lines exhibit higher sensitivity to JK184 treatment compared to MCF10a, MTSV1-7, or HMLE-shGFP and HMLE-pBP cells. JK184 induces a dose-dependent decrease in glioma-associated oncogene homolog 1 (GLI1) transcript and protein levels in these cells. Treatment with the IC50 dose of JK184 increases the proportion of HMLE-shEcad cells staining positive for Annexin-V but negative for propidium iodide (PI) (P<0.0001)[2].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.85mL

0.57mL

0.29mL

14.27mL

2.85mL

1.43mL

28.54mL

5.71mL

2.85mL
参考文献

[1]Zhang N, et al. Biodegradable polymeric micelles encapsulated JK184 suppress tumor growth through inhibiting Hedgehog signaling pathway. Nanoscale. 2015 Feb 14;7(6):2609-24.

[2]Colavito SA, et al. Significance of glioma-associated oncogene homolog 1 (GLI1) expression in claudin-low breast cancer and crosstalk with the nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB) pathway. Breast Cancer Res. 2014 Sep 25;16