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SH-4-54

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Chemical Structure| 1456632-40-8 同义名 : -
CAS号 : 1456632-40-8
货号 : A627552
分子式 : C29H27F5N2O5S
纯度 : 99%+
分子量 : 610.592
MDL号 : MFCD28143917
存储条件：

粉末 Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(171.96 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
靶点

  • STAT3

    STAT3, Kd:300 nM

  • STAT5

    STAT5, Kd:464 nM
描述 SH-4-54 demonstrates remarkable effectiveness in killing glioblastoma brain cancer stem cells (BTSCs) by strongly inhibiting STAT3 phosphorylation and its downstream transcriptional activities at low nanomolar concentrations. Exhibiting significant cytotoxicity against human BTSCs while sparing human fetal astrocytes, SH-4-54 effectively inhibits pSTAT3 with nanomolar IC50s, showing specific action without detectable off-target effects at therapeutic levels[1].
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
human 127EF cells Cytotoxic assay 3 days Cytotoxicity against human 127EF cells assessed as cell viability after 3 days by Alamar Blue assay, IC50=66 nM 24900612
human 147EF cells Function assay 2-72 h Inhibition of STAT3 in human 147EF cells assessed as reduction of phosphorylated cyclin D1 level after 2 to 72 hrs by Western blotting analysis 24900612
human 25EF cells Cytotoxic assay 3 days Cytotoxicity against human 25EF cells assessed as cell viability after 3 days by Alamar Blue assay, IC50=0.234 μM 24900612
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.64mL

0.33mL

0.16mL

8.19mL

1.64mL

0.82mL

16.38mL

3.28mL

1.64mL
参考文献

[1]Haftchenary S, et al. Potent Targeting of the STAT3 Protein in Brain Cancer Stem Cells: A Promising Route for Treating Glioblastoma. ACS Med Chem Lett. 2013 Sep 8;4(11):1102-1107.