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(±)-Stylopine

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Chemical Structure| 4312-32-7 同义名 : 四氢黄莲碱 ;Tetrahydrocoptisine;NSC 110382;(R,S±)-Stylopine;NSC 404529;Stylopine
CAS号 : 4312-32-7
货号 : A614191
分子式 : C19H17NO4
纯度 : 99%+
分子量 : 323.343
MDL号 : MFCD00075989
存储条件：

粉末 Inert atmosphere,Room Temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 2 mg/mL(6.19 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

DMF: 4 mg/mL(12.37 mM)，配合低频超声，并水浴加热至45℃助溶
动物实验配方：
生物活性
描述 Tetrahydrocoptisine (THC) is a natural alkaloid compound isolated and purified from Corydalis tubers with anti-inflammatory and anti-parasitic activities. Pretreatment of THC at doses of 10 and 20mg/kg bodyweight significantly attenuated the gastric lesions as compared to the ethanol group. The gastroprotective activity of THC is attributed to reducing NO production and adjusting the pro-inflammatory cytokine, inhibited neutrophil accumulation and NF-κB expression[3]. THC significantly inhibited LPS-induced TNF-α, interleukin-6(IL-6) and nitric oxide (NO) production. THC inhibited the production of TNF-α and IL-6 by down-regulating LPS-induced IL-6 and TNF-α mRNA expression. Furthermore, it attenuated the phosphorylation of p38 mitogen-activated protein kinase (p38MAPK) and phosphorylation of extracellular signal-regulated kinase1/2 (ERK1/2) as well as the expression of nuclear factor kappa B(NF-κB), in a concentration-dependent manner[4]. Tetrahydrocoptisine possesses a protective effect on LPS-induced ALI (acute lung injury) through inhibiting of NF-κB signaling pathways, which may involve the inhibition of pulmonary inflammatory process[5].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.09mL

0.62mL

0.31mL

15.46mL

3.09mL

1.55mL

30.93mL

6.19mL

3.09mL
参考文献

[1]Li W, Huang H, et al. Protective effect of tetrahydrocoptisine against ethanol-induced gastric ulcer in mice. Toxicol Appl Pharmacol. 2013 Oct 1;272(1):21-9.

[2]Li W, Huang H, et al. Anti-inflammatory effect of tetrahydrocoptisine from Corydalis impatiens is a function of possible inhibition of TNF-α, IL-6 and NO production in lipopolysaccharide-stimulated peritoneal macrophages through inhibiting NF-κB activation and MAPK pathway. Eur J Pharmacol. 2013 Sep 5;715(1-3):62-71.

[3]Li W, Huang H, Niu X, Fan T, Mu Q, Li H. Protective effect of tetrahydrocoptisine against ethanol-induced gastric ulcer in mice. Toxicol Appl Pharmacol. 2013 Oct 1;272(1):21-9

[4]Li W, Huang H, Zhang Y, Fan T, Liu X, Xing W, Niu X. Anti-inflammatory effect of tetrahydrocoptisine from Corydalis impatiens is a function of possible inhibition of TNF-α, IL-6 and NO production in lipopolysaccharide-stimulated peritoneal macrophages through inhibiting NF-κB activation and MAPK pathway. Eur J Pharmacol. 2013 Sep 5;715(1-3):62-71

[5]Li W, Huang H, Niu X, Fan T, Hu H, Li Y, Yao H, Li H, Mu Q. Tetrahydrocoptisine protects rats from LPS-induced acute lung injury. Inflammation. 2014 Dec;37(6):2106-15