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Dencichine

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Chemical Structure| 5302-45-4 同义名 : ODAP;Dencichin;Ox-Dapro;BRN 2259036;β-ODAP
CAS号 : 5302-45-4
货号 : A612304
分子式 : C5H8N2O5
纯度 : 98%
分子量 : 176.127
MDL号 : MFCD00055228
存储条件：

粉末 Keep in dark place,Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 4 mg/mL(22.71 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 5 mg/mL(28.39 mM)，配合低频超声助溶

0.1 M HCL: 3 mg/mL(17.03 mM)，配合低频超声，并水浴加热至45℃助溶
动物实验配方：
生物活性
描述 β-ODAP is a strong agonist of AMPA (alpha-amino-3-hydroxy-5-methylisoxazole-4-proprionate) receptors, the potency being almost the same as l-glutamate[3]. 3-N-Oxalyl-l-2,3-diaminopropanoic acid (β-ODAP) induces neurolathyrism, a motor neuron disease. β-ODAP had little effect on the glutamate-evoked currents through the expressed NMDA receptor (NR1(A)/NR2A), but showed a weak inhibitory effect on the glycine-modulatory site[4]. L-β-ODAP (β-ODAP) caused a prolonged rise of intracellular Ca(2+) ([Ca(2+)]i) in rat spinal cord MNs, and the [Ca(2+)]i accumulation was inversely proportional to the MN's life span[5]. β-ODAP is a neuroexcitatory non-protein amino acid and presents in the seeds of the hardy legume crop grass pea (Lathyrus sativus L.), was considered responsible for human lathyrism[6]. beta-ODAP could supply nitrogen for plant growth and seed development, scavenge reactive oxygen species (ROS), involve in osmotic adjustment as a soluble amino acid, transport zinc-ions as a carrier molecule, and impact nodule development. Although beta-ODAP could induce excitotoxicity by damaging intracellular Ca2+ homeostasis and as glutamate analogues, it has medicinal value on hemostasis and anti-tumor[7].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

5.68mL

1.14mL

0.57mL

28.39mL

5.68mL

2.84mL

56.78mL

11.36mL

5.68mL
参考文献

[1]Eslavath RK, Sharma D, et al. β-N-oxalyl-L-α, β- diaminopropionic acid induces HRE expression by inhibiting HIF-prolyl hydroxylase-2 in normoxic conditions. Eur J Pharmacol. 2016 Nov 15;791:405-411.

[2]Jammulamadaka N, Burgula S, et al. β-N-oxalyl-L-α,β-diaminopropionic acid regulates mitogen-activated protein kinase signaling by down-regulation of phosphatidylethanolamine-binding protein 1. J Neurochem. 2011 Jul;118(2):176-86.

[3]Lin CY, Lynch G, Gall CM. AMPA receptor stimulation increases alpha5beta1 integrin surface expression, adhesive function and signaling. J Neurochem. 2005 Jul;94(2):531-46

[4]Kusama-Eguchi K, Ikegami F, Kusama T, Lambein F, Watanabe K. Effects of β-ODAP and its biosynthetic precursor on the electrophysiological activity of cloned glutamate receptors. Environ Toxicol Pharmacol. 1996 Dec 20;2(4):339-42

[5]Kusama-Eguchi K, Miyano T, Yamamoto M, Suda A, Ito Y, Ishige K, Ishii M, Ogawa Y, Watanabe K, Ikegami F, Kusama T. New insights into the mechanism of neurolathyrism: L-β-ODAP triggers [Ca2+]i accumulation and cell death in primary motor neurons through transient receptor potential channels and metabotropic glutamate receptors. Food Chem Toxicol. 2014 May;67:113-22

[6]Jiao CJ, Jiang JL, Ke LM, Cheng W, Li FM, Li ZX, Wang CY. Factors affecting β-ODAP content in Lathyrus sativus and their possible physiological mechanisms. Food Chem Toxicol. 2011 Mar;49(3):543-9

[7]Xiong JL, Bai X, Batool A, Kong HY, Tan RY, Wang YF, Li ZX, Xiong YC. [Ecological function and application of toxin beta-ODAP in grass pea (Lathyrus sativus)]. Ying Yong Sheng Tai Xue Bao. 2014 Apr;25(4):1197-205. Chinese