产品说明书
DTT
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同义名 : | DTT |
| CAS号 : | 3483-12-3 | |
| 货号 : | A587635 | |
| 分子式 : | C4H10O2S2 | |
| 纯度 : | 95% | |
| 分子量 : | 154.25 | |
| MDL号 : | MFCD00004877 | |
| 存储条件： |
粉末 Inert atmosphere,2-8°C 液体 -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 105 mg/mL(680.71 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO H2O: 200 mg/mL(1296.59 mM) |
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| 动物实验配方： |
| 生物活性 | |||
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| 描述 | DTT is a reducing agent. DTT forms a stable six-membered ring with an internal disulfide bond once oxidized. DTT is widely used in biochemistry works to reduce dissulfide bridges, protect biomolecules, in sample preparation, and to denature proteins before electrophoresis analysis (SDS-PAGE). The thiol-containing compound Dithiothreitol (DTT) has been shown to be toxic to cultured cells by inducing the generation of reactive oxygen species that ultimately cause cell death[1]. Daratumumab (DARA) consistently interferes with routine blood bank serologic testing by directly binding to CD38 expressed on reagent red blood cells (RBCs). Treating RBCs with dithiothreitol (DTT) eliminates the DARA interference. The DTT method is robust and reproducible and can be implemented by transfusion services worldwide to help provide safe blood products to patients treated with DARA (Daratumumab)[2]. The dithiothreitol (DTT) assay is the most commonly used method to quantify the oxidative potential of fine particles[3]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
6.48mL 1.30mL 0.65mL |
32.41mL 6.48mL 3.24mL |
64.83mL 12.97mL 6.48mL |
| 参考文献 |
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