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Wogonin

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Chemical Structure| 632-85-9 同义名 : 汉黄芩黄酮 ;BRN 0287152;Vogonin
CAS号 : 632-85-9
货号 : A583686
分子式 : C16H12O5
纯度 : 99%+
分子量 : 284.264
MDL号 : MFCD00017736
存储条件：

粉末 Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 60 mg/mL(211.07 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
靶点

  • CDK9
描述 Wogonin, a naturally occurring flavonoid derived from the root extract of Scutellaria baicalensis, used in the treatment of inflammatory diseases. In vivo, treatment of osteoarthritis chondrocytes with Wogonin (10-50 μM) significantly blocked the IL-1β induced production of NO in a dose dependent manner[3]. Moreover, wogonin protected retinal ganglion cells survival and suppressed neuro-inflammation in retina after optic nerve crush by inhibiting TLR4-NF-κB pathways[4]. In vitro, DNA intercalation by Wogonin may result in the stabilization of genomic DNA leading to protective activity[6].
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
RAW264.7 cells Function assay Inhibition of COX2-mediated PGE2 production in LPS-stimulated mouse RAW264.7 cells by enzyme immunoassay, IC50=1.07 μM 19233646
SW1353 cells Function assay Antiinflammatory activity in PMA-stimulated human SW1353 cells assessed as inhibition of NF-kappaB activation, IC50=39 μM 19555121
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.52mL

0.70mL

0.35mL

17.59mL

3.52mL

1.76mL

35.18mL

7.04mL

3.52mL
参考文献

[1]Zhu Y, Wang J. Wogonin increases β-amyloid clearance and inhibits tau phosphorylation via inhibition of mammalian target of rapamycin: potential drug to treat Alzheimer's disease. Neurol Sci. 2015 Jul;36(7):1181-8.

[2]He L, Lu N, et al. Wogonin induced G1 cell cycle arrest by regulating Wnt/β-catenin signaling pathway and inactivating CDK8 in human colorectal cancer carcinoma cells. Toxicology. 2013 Oct 4;312:36-47.

[3]Khan NM, Haseeb A, Ansari MY, Devarapalli P, Haynie S, Haqqi TM. Wogonin, a plant derived small molecule, exerts potent anti-inflammatory and chondroprotective effects through the activation of ROS/ERK/Nrf2 signaling pathways in human Osteoarthritis chondrocytes. Free Radic Biol Med. 2017 May;106:288-301. doi: 10.1016/j.freeradbiomed.2017.02.041. Epub 2017 Feb 22. PMID: 28237856; PMCID: PMC5490997.

[4]Xu Y, Yang B, Hu Y, Lu L, Lu X, Wang J, Xu F, Yu S, Huang J, Liang X. Wogonin prevents TLR4-NF-κB-medicated neuro-inflammation and improves retinal ganglion cells survival in retina after optic nerve crush. Oncotarget. 2016 Nov 8;7(45):72503-72517. doi: 10.18632/oncotarget.12700. PMID: 27756890; PMCID: PMC5341925.

[5]Wu X, Liu P, Zhang H, et al. Wogonin as a targeted therapeutic agent for EBV (+) lymphoma cells involved in LMP1/NF-κB/miR-155/PU.1 pathway. BMC Cancer. 2017;17(1):147. Published 2017 Feb 21.

[6]Khan NM, Ahmad I, Ansari MY, Haqqi TM. Wogonin, a natural flavonoid, intercalates with genomic DNA and exhibits protective effects in IL-1β stimulated osteoarthritis chondrocytes. Chem Biol Interact. 2017 Aug 25;274:13-23. doi: 10.1016/j.cbi.2017.06.025. Epub 2017 Jul 5. PMID: 28688942; PMCID: PMC5548605.