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NBQX

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Chemical Structure| 118876-58-7 同义名 : FG9202;NNC 079202
CAS号 : 118876-58-7
货号 : A546099
分子式 : C12H8N4O6S
纯度 : 99%+
分子量 : 336.28
MDL号 : MFCD00270054
存储条件：

粉末 Keep in dark place,Inert atmosphere,Room temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 80 mg/mL(237.9 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 The hippocampus is an important brain region that is involved in neurological disorders such as Alzheimer disease, schizophrenia, and epilepsy. Ionotropic glutamate receptors—namely, N-methyl-D-aspartate (NMDA) receptors (NMDARs), α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptors (AMPARs), and kainic acid (KA) receptors (KARs)—are well known to be involved in these diseases by mediating long-term potentiation, excitotoxicity, or both. NBQX is an antagonist of AMPARs and KARs. NBQX for AMPA and KA stimulation (IC50 = 0.7 ± 0.1 and 0.7 ± 0.03 μM, respectively; n = 3). The effects of NBQX on the AMPA- or KA-evoked calcium rise in differentiated HIP-009 cells were assessed. NBQX inhibited both AMPA- and KA-induced signals in a concentration-dependent fashion (IC50 = 0.7 ± 0.1 and 0.7 ± 0.03 μM for AMPA and KA stimulation, respectively, n = 3). The AMPA-evoked calcium rise was completely inhibited by NBQX, whereas 68.6% ± 1.3% inhibition of the KA-induced signal was observed with 30 μM of NBQX treatment. The effect of co-treatment with MK-801 (a NMDAR antagonist) and NBQX on the glutamate-evoked calcium rise was assessed. When 30 μM of MK-801 or NBQX was added alone, 44.5% ± 2.2% or 34.2% ± 5.0%, respectively, of the glutamate-induced calcium rise was inhibited. Co-treatment had an additive inhibitory effect on total calcium rise upon glutamate stimulation (78.6% ± 2.2% inhibition)[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.97mL

0.59mL

0.30mL

14.87mL

2.97mL

1.49mL

29.74mL

5.95mL

2.97mL
参考文献

[1]Fukushima K, Tabata Y, Imaizumi Y, et al. Characterization of Human Hippocampal Neural Stem/Progenitor Cells and Their Application to Physiologically Relevant Assays for Multiple Ionotropic Glutamate Receptors. J Biomol Screen. 2014;19(8):1174-1184.