GSK 3 Inhibitor IX

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Chemical Structure| 667463-62-9 同义名 : 6-Bromoindirubin-3'-oxime;MLS 2052;​GSK3 Inhibitor IX;6-bromoindirubin-3-oxime;BIO
CAS号 : 667463-62-9
货号 : A538818
分子式 : C16H10BrN3O2
纯度 : 99%+
分子量 : 356.173
MDL号 : MFCD08705318
存储条件：

Pure form Keep in dark place,Inert atmosphere,2-8°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 20 mg/mL(56.15 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：

5% DMSO+40% PEG 300+5% Tween 80+50%water 3 mg/mL

生物活性
靶点

  • GSK-3

    GSK-3, IC50:5 nM
描述 BIO is a potent and selective inhibitor of GSK-3 and CDK1–cyclinB complex with IC50 of 5 nM/320 nM/83 nM for GSK-3αβ/CDK1/CDK5 respectively.
细胞研究
细胞系 浓度 检测类型 检测时间 活性说明 数据源
HEK293 cells 0.5-1 μM Function assay Inhibition of human GSK3 activity in HEK293 cells containing the Wnt/beta-catenin activated reporter pSuperTOPFLASH (STF293 cells) at 0.5 to 1 uM by Wnt reporter gene assay 24697244
human HCT116 cells Proliferation assay 72 h Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay, IC50=5.2 μM 19783149
human HL60 cells Proliferation assay 5 days Antiproliferative activity against human HL60 cells after 5 days by MTT assay 19783149
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.81mL

0.56mL

0.28mL

14.04mL

2.81mL

1.40mL

28.08mL

5.62mL

2.81mL
参考文献

[1]Liu L, Nam S, et al. 6-Bromoindirubin-3'-oxime inhibits JAK/STAT3 signaling and induces apoptosis of human melanoma cells. Cancer Res. 2011 Jun 1;71(11):3972-9.

[2]Meijer L, Skaltsounis AL, et al. GSK-3-selective inhibitors derived from Tyrian purple indirubins. Chem Biol. 2003 Dec;10(12):1255-66.