PD 169316

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Chemical Structure| 152121-53-4 同义名 : -
CAS号 : 152121-53-4
货号 : A530681
分子式 : C20H13FN4O2
纯度 : 99%+
分子量 : 360.341
MDL号 : MFCD12405019
存储条件：

Pure form Keep in dark place,Sealed in dry,2-8°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 12 mg/mL(33.3 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 PD 169316 is a potent and selective p38 MAP kinase inhibitor. It blocked p38 enzyme activity activated by serum-starvation and apoptosis by approximately 80% in Rat-1 fibroblasts at concentration of 10μM. PD169316 at 5μM or higher abrogated signaling initiated by both TGFβ and Activin A, but not BMP4, in CaOV3 cells. PD169316 at 10μM blocked the apoptotic process induced upon LIF withdrawal, without impairing cell differentiation, in mouse ES cells, with decreased FGF-5 and increased Bcl-2 expression on RNA level observed post 3 days.
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.78mL

0.56mL

0.28mL

13.88mL

2.78mL

1.39mL

27.75mL

5.55mL

2.78mL
参考文献

[1]Kummer JL, Rao PK, et al. Apoptosis induced by withdrawal of trophic factors is mediated by p38 mitogen-activated protein kinase. J Biol Chem. 1997 Aug 15;272(33):20490-4.

[2]Fu Y, O'Connor LM, et al. The p38 MAPK inhibitor, PD169316, inhibits transforming growth factor beta-induced Smad signaling in human ovarian cancer cells. Biochem Biophys Res Commun. 2003 Oct 17;310(2):391-7.

[3]Duval D, Malaisé M, et al. A p38 inhibitor allows to dissociate differentiation and apoptotic processes triggered upon LIF withdrawal in mouse embryonic stem cells. Cell Death Differ. 2004 Mar;11(3):331-41.